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Literature DB >> 8264566

Determination of phosphotyrosine phosphatase (PTPase) activity in normal and transformed cells.

Abstract

Through the development of a new colorimetric and non-radioactive method it is possible to measure PTPase activity in cell extracts. Applying this method we were able to demonstrate that intracellular PTPase activity in chicken embryo fibroblasts increases significantly after RSV induced transformation of these cells. PTPase activity in normal and transformed cells is inhibited by microM concentrations of vanadate, molybdate and zinc ion, but is not affected by mM concentrations of calcium, magnesium and sodium fluoride. The transformation specific activation of the PTPase seems to represent an early parameter during the events of cellular transformation.

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Year: 1993 PMID： 8264566 DOI： 10.1007/bf00926828

Source DB: PubMed Journal: Mol Cell Biochem ISSN： 0300-8177 Impact factor: 3.396

23 in total

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Journal: Mol Cell Biol Date: 1984-06 Impact factor: 4.272

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Journal: Cell Date: 1981-04 Impact factor: 41.582

6. Simultaneous injection of newborn rabbits with the Schmidt-Ruppin and prague strains of Rous sarcoma virus induces antibodies which recognize the pp60src of both strains.

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Journal: J Gen Virol Date: 1980-09 Impact factor: 3.891

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Journal: Proc Natl Acad Sci U S A Date: 1980-03 Impact factor: 11.205

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Authors: A A Baykov; O A Evtushenko; S M Avaeva
Journal: Anal Biochem Date: 1988-06 Impact factor: 3.365

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Authors: A Barnekow; H Bauer
Journal: Biochim Biophys Acta Date: 1984-05-15

10. Regulation of protein serine-threonine phosphatase type-2A by tyrosine phosphorylation.

Authors: J Chen; B L Martin; D L Brautigan
Journal: Science Date: 1992-08-28 Impact factor: 47.728

1 in total

1. Insulin-like effects of tungstate and molybdate: mediation through insulin receptor independent pathways.

Authors: J Li; G Elberg; J Libman; A Shanzer; D Cefel; Y Shechter
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1 in total