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Literature DB >> 8264545

Determination of the size of the Azotobacter vinelandii chromosome.

Abstract

The chromosome of Azotobacter vinelandii UW was digested separately with the rape cutter restriction endonucleases SwaI (5'-ATTTAAAT), PmeI (5'GTTTAAAC) and PacI (5'-TTAATTAA) and the products were separated by pulsed-field gel electrophoresis. The size of the chromosome was determined to be approximately 4.5 megabase pairs (Mb) based on the sum of the sizes of the restriction fragments. This is almost the same as the size of the chromosome of Escherichia coli. The inability of the undigested DNA to enter the gel has led us to infer that the chromosome is circular.

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Year: 1993 PMID： 8264545 DOI： 10.1007/bf00279916

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

13 in total

1. A chromosomal linkage map of Azotobacter vinelandii.

Authors: G Blanco; F Ramos; J R Medina; M Tortolero
Journal: Mol Gen Genet Date: 1990-11

2. NotI genomic cleavage map of Escherichia coli K-12 strain MG1655.

Authors: J D Heath; J D Perkins; B Sharma; G M Weinstock
Journal: J Bacteriol Date: 1992-01 Impact factor: 3.490

3. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.

Authors: Y Kohara; K Akiyama; K Isono
Journal: Cell Date: 1987-07-31 Impact factor: 41.582

Determination of the size of the Azotobacter vinelandii chromosome.

1. A chromosomal linkage map of Azotobacter vinelandii.

2. NotI genomic cleavage map of Escherichia coli K-12 strain MG1655.

3. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.

4. Multiple chromosomes of Azotobacter vinelandii.

5. A physical map of the Escherichia coli K12 genome.

6. Formation of the nitrogen-fixing enzyme system in Azotobacter vinelandii.

7. Separation of large DNA molecules by contour-clamped homogeneous electric fields.

8. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

9. Characterization of Azotobacter vinelandii deoxyribonucleic acid and folded chromosomes.

10. Timing of initiation of chromosome replication in individual Escherichia coli cells.

1. Changes of ploidy during the Azotobacter vinelandii growth cycle.

2. Characterization of the gene coding for GDP-mannose dehydrogenase (algD) from Azotobacter vinelandii.