| Literature DB >> 8258994 |
M Steinitz1, A Livoff, S Tamir, T Brenner.
Abstract
A universal method for selection of surface marker-positive cells is described. The cells, admixed with an excess of surface marker-negative cells, are first labelled with a specific biotinylated ligand and then isolated with the aid of monoclonal, anti-biotin coated beads. The method enables selection and isolation of cells with a frequency as low as 10(-4). The ligand can be an antigen (for selection of infrequent antibody-producing cells), an antibody (for selection of surface antigen-positive cells) or other molecules (for selection of specific receptor-positive cells).Entities:
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Year: 1993 PMID： 8258994 DOI： 10.1007/bf02987768
Source DB: PubMed Journal: Med Oncol Tumor Pharmacother ISSN： 0736-0118