Literature DB >> 8254748

Synthesis of viruslike particles by expression of the putative capsid protein of Leishmania RNA virus in a recombinant baculovirus expression system.

T L Cadd1, J L Patterson.   

Abstract

The putative capsid open reading frame (ORF2) of the Leishmania RNA virus LRV1-4 was expressed in a baculovirus expression system. The expressed protein was identified by Western immunoblot analysis with polyclonal antiserum raised to purified LRV1-4 virus. Electron microscopy and sedimentation analysis indicated that the expressed protein self-assembles into empty viruslike particles of similar size and shape to authentic virus particles, thus confirming that ORF2 encodes the viral capsid. The expressed particles are present exclusively in the cytoplasm of infected SF9 cells and are able to assemble in the absence of LRV1-4 RNA, viral polymerase, or any Leishmania host factors.

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Year:  1994        PMID： 8254748      PMCID： PMC236296     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  23 in total

1.  Expression, self-assembly, and antigenicity of the Norwalk virus capsid protein.

Authors:  X Jiang; M Wang; D Y Graham; M K Estes
Journal:  J Virol       Date:  1992-11       Impact factor: 5.103

Review 2.  Protozoal viruses.

Authors:  L S Diamond; C F Mattern
Journal:  Adv Virus Res       Date:  1976       Impact factor: 9.937

3.  LR1: a candidate RNA virus of Leishmania.

Authors:  P I Tarr; R F Aline; B L Smiley; J Scholler; J Keithly; K Stuart
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205

4.  Electron microscopy of an antibody-hapten complex.

Authors:  R C Valentine; N M Green
Journal:  J Mol Biol       Date:  1967-08-14       Impact factor: 5.469

5.  Detection of Leishmania RNA virus 1 proteins.

Authors:  T L Cadd; M C Keenan; J L Patterson
Journal:  J Virol       Date:  1993-09       Impact factor: 5.103

6.  MAK3 encodes an N-acetyltransferase whose modification of the L-A gag NH2 terminus is necessary for virus particle assembly.

Authors:  J C Tercero; R B Wickner
Journal:  J Biol Chem       Date:  1992-10-05       Impact factor: 5.157

7.  Successful transient introduction of Leishmania RNA virus into a virally infected and an uninfected strain of Leishmania.

Authors:  T C Armstrong; M C Keenan; G Widmer; J L Patterson
Journal:  Proc Natl Acad Sci U S A       Date:  1993-03-01       Impact factor: 11.205

8.  Expression of a bacterial gene in a trypanosomatid protozoan.

Authors:  V Bellofatto; G A Cross
Journal:  Science       Date:  1989-06-09       Impact factor: 47.728

9.  Characterization of a RNA virus from the parasite Leishmania.

Authors:  G Widmer; A M Comeau; D B Furlong; D F Wirth; J L Patterson
Journal:  Proc Natl Acad Sci U S A       Date:  1989-08       Impact factor: 11.205

10.  The double-stranded RNA genome of yeast virus L-A encodes its own putative RNA polymerase by fusing two open reading frames.

Authors:  T Icho; R B Wickner
Journal:  J Biol Chem       Date:  1989-04-25       Impact factor: 5.157
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  15 in total

1.  Identification of the minimal essential RNA sequences responsible for site-specific targeting of the Leishmania RNA virus 1-4 capsid endoribonuclease.

Authors:  Y T Ro; J L Patterson
Journal:  J Virol       Date:  2000-01       Impact factor: 5.103

2.  Concentration of 2'C-methyladenosine triphosphate by Leishmania guyanensis enables specific inhibition of Leishmania RNA virus 1 via its RNA polymerase.

Authors:  John I Robinson; Stephen M Beverley
Journal:  J Biol Chem       Date:  2018-03-06       Impact factor: 5.157

3.  Hygromycin B resistance mediates elimination of Leishmania virus from persistently infected parasites.

Authors:  Y T Ro; S M Scheffter; J L Patterson
Journal:  J Virol       Date:  1997-12       Impact factor: 5.103

4.  Specific in vitro cleavage of a Leishmania virus capsid-RNA-dependent RNA polymerase polyprotein by a host cysteine-like protease.

Authors:  Y T Ro; S M Scheffter; J L Patterson
Journal:  J Virol       Date:  1997-12       Impact factor: 5.103

5.  Single-site cleavage in the 5'-untranslated region of Leishmaniavirus RNA is mediated by the viral capsid protein.

Authors:  K J MacBeth; J L Patterson
Journal:  Proc Natl Acad Sci U S A       Date:  1995-09-12       Impact factor: 11.205

6.  Characterization of virus-like particles produced by the expression of rotavirus capsid proteins in insect cells.

Authors:  S E Crawford; M Labbé; J Cohen; M H Burroughs; Y J Zhou; M K Estes
Journal:  J Virol       Date:  1994-09       Impact factor: 5.103

7.  Mutational analysis of the capsid protein of Leishmania RNA virus LRV1-4.

Authors:  T L Cadd; K MacBeth; D Furlong; J L Patterson
Journal:  J Virol       Date:  1994-12       Impact factor: 5.103

8.  Purification, identification, and biochemical characterization of a host-encoded cysteine protease that cleaves a leishmaniavirus gag-pol polyprotein.

Authors:  Ricardo Carrion; Young-Tae Ro; Jean L Patterson
Journal:  J Virol       Date:  2003-10       Impact factor: 5.103

9.  Phylogenetic analysis of Leishmania RNA virus and Leishmania suggests ancient virus-parasite association.

Authors:  G Widmer; S Dooley
Journal:  Nucleic Acids Res       Date:  1995-06-25       Impact factor: 16.971

10.  Suppression of Leishmania RNA virus replication by capsid protein overexpression.

Authors:  G Widmer
Journal:  J Virol       Date:  1995-07       Impact factor: 5.103
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