Literature DB >> 8244021

Cloning and sequence analysis of the gene encoding a thermostable chitinase from Streptomyces thermoviolaceus OPC-520.

H Tsujibo1, H Endo, K Minoura, K Miyamoto, Y Inamori.   

Abstract

The gene (chi40) encoding a thermostable chitinase from Streptomyces thermoviolaceus OPC-520 was cloned in Escherichia coli JM109 using pUC18. The nucleotide (nt) sequence of chi40 has been determined. A single open reading frame (ORF) encoded a protein consisting of 414 amino acids (aa) with a M(r) of 43,838. The deduced aa sequence of the cloned chitinase (Chi40) showed striking homology (74%) with Chi63 from Streptomyces plicatus. Comparison with other chitinases revealed that Chi40 contained the two conserved regions common to microbial and plant chitinases. Furthermore, the putative promoter region of chi40, which might be involved in the repression and induction of such catabolite-controlled genes, was detected by the DNA sequence alignments.

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Year:  1993        PMID： 8244021     DOI： 10.1016/0378-1119(93)90183-4

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  13 in total

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2.  Characterization of chitinase C from a marine bacterium, Alteromonas sp. strain O-7, and its corresponding gene and domain structure.

Authors:  H Tsujibo; H Orikoshi; K Shiotani; M Hayashi; J Umeda; K Miyamoto; C Imada; Y Okami; Y Inamori
Journal:  Appl Environ Microbiol       Date:  1998-02       Impact factor: 4.792

3.  Direct repeat sequences in the Streptomyces chitinase-63 promoter direct both glucose repression and chitin induction.

Authors:  X Ni; J Westpheling
Journal:  Proc Natl Acad Sci U S A       Date:  1997-11-25       Impact factor: 11.205

4.  glkA is involved in glucose repression of chitinase production in Streptomyces lividans.

Authors:  A Saito; T Fujii; T Yoneyama; K Miyashita
Journal:  J Bacteriol       Date:  1998-06       Impact factor: 3.490

5.  Purification and characterization of three thermostable endochitinases of a noble Bacillus strain, MH-1, isolated from chitin-containing compost.

Authors:  K Sakai; A Yokota; H Kurokawa; M Wakayama; M Moriguchi
Journal:  Appl Environ Microbiol       Date:  1998-09       Impact factor: 4.792

6.  Characterization of chitinase genes from an alkaliphilic actinomycete, Nocardiopsis prasina OPC-131.

Authors:  Hiroshi Tsujibo; Takahiro Kubota; Mitsugu Yamamoto; Katsushiro Miyamoto; Yoshihiko Inamori
Journal:  Appl Environ Microbiol       Date:  2003-02       Impact factor: 4.792

7.  Partial characterization of the Streptomyces lividans xlnB promoter and its use for expression of a thermostable xylanase from Thermotoga maritima.

Authors:  C C Chen; J Westpheling
Journal:  Appl Environ Microbiol       Date:  1998-11       Impact factor: 4.792

8.  Physiological Studies of Cellulase (Avicelase) Synthesis in Streptomyces reticuli.

Authors:  S Walter; H Schrempf
Journal:  Appl Environ Microbiol       Date:  1996-03       Impact factor: 4.792

9.  A novel beta-N-acetylglucosaminidase from Streptomyces thermoviolaceus OPC-520: gene cloning, expression, and assignment to family 3 of the glycosyl hydrolases.

Authors:  H Tsujibo; N Hatano; T Mikami; A Hirasawa; K Miyamoto; Y Inamori
Journal:  Appl Environ Microbiol       Date:  1998-08       Impact factor: 4.792

10.  Characterization and phylogeny of the pfp gene of Amycolatopsis methanolica encoding PPi-dependent phosphofructokinase.

Authors:  A M Alves; W G Meijer; J W Vrijbloed; L Dijkhuizen
Journal:  J Bacteriol       Date:  1996-01       Impact factor: 3.490
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