Nucleotide sequence and E. coli expression of the coat protein gene of the yellowing strain of soybean dwarf luteovirus.

The nucleotide sequence of the coat protein gene of the yellowing (Y) strain of soybean dwarf virus (SbDV) was determined from cloned cDNA. The gene contains 600 nucleotides and encodes a protein of 200 amino acids with a calculated M(r) of 22,200. The identity of the open reading frame (ORF) encoding the coat protein was confirmed by expression of an Escherichia coli beta-galactosidase fusion protein and detection by a dot blot immunoassay. Sequence comparisons of the deduced coat protein amino acids to several luteoviruses demonstrated that the SbDV-Y coat protein ORF shares greatest similarity with bean leaf roll virus (BLRV) (65%).