OBJECTIVE: To examine the genetic heterogeneity of the V3 region of HIV-1 gp120 from 22 Brazilian HIV-1 specimens. DESIGN: Genetic heterogeneity was examined by DNA sequencing of the C2 V3 region of the HIV-1 envelope (env) gene from polymerase chain reaction (PCR)-amplified HIV-1 DNA. Deduced amino-acid sequences were compared to determine the extent of amino-acid conservation among the Brazilian specimens. Genetic similarity among and between the Brazilian specimens and other previously published HIV-1 isolates was analyzed by principal co-ordinate and DNA parsimony methods. METHODS: A 282 base pair (bp) region of a 1.5 kilo (k) bp PCR-amplified HIV-1 env fragment was sequenced by a Taq dye-labeled primer cycle sequencing reaction. Nucleotide sequences were used to analyze inter-specimen relationships based on overall nucleotide sequence similarity and DNA parsimony principles. RESULTS: Amino-acid comparison showed that 15 of the 35 (43%) residues of the V3 loop were conserved among the Brazilian specimens. Nine of the 22 (40%) Brazilian specimens contained the North American-European GPGR tetrapeptide motif, while eight (36%) contained the GWGR motif, previously reported in Japanese isolates. Principal co-ordinate analysis demonstrated that 19 of the 20 examined Brazilian HIV-1 specimens were more similar to North American and Haitian isolates than to African isolates. Similar results were also obtained by DNA parsimony analysis. CONCLUSION: The majority of the Brazilian specimens examined are more genetically related to North American and Haitian HIV-1 isolates than to African isolates. This finding and the presence of a GWGR V3 loop motif in some Brazilian isolates may be important for vaccine development.
OBJECTIVE: To examine the genetic heterogeneity of the V3 region of HIV-1gp120 from 22 Brazilian HIV-1 specimens. DESIGN: Genetic heterogeneity was examined by DNA sequencing of the C2 V3 region of the HIV-1envelope (env) gene from polymerase chain reaction (PCR)-amplified HIV-1 DNA. Deduced amino-acid sequences were compared to determine the extent of amino-acid conservation among the Brazilian specimens. Genetic similarity among and between the Brazilian specimens and other previously published HIV-1 isolates was analyzed by principal co-ordinate and DNA parsimony methods. METHODS: A 282 base pair (bp) region of a 1.5 kilo (k) bp PCR-amplified HIV-1env fragment was sequenced by a Taq dye-labeled primer cycle sequencing reaction. Nucleotide sequences were used to analyze inter-specimen relationships based on overall nucleotide sequence similarity and DNA parsimony principles. RESULTS: Amino-acid comparison showed that 15 of the 35 (43%) residues of the V3 loop were conserved among the Brazilian specimens. Nine of the 22 (40%) Brazilian specimens contained the North American-European GPGR tetrapeptide motif, while eight (36%) contained the GWGR motif, previously reported in Japanese isolates. Principal co-ordinate analysis demonstrated that 19 of the 20 examined Brazilian HIV-1 specimens were more similar to North American and Haitian isolates than to African isolates. Similar results were also obtained by DNA parsimony analysis. CONCLUSION: The majority of the Brazilian specimens examined are more genetically related to North American and Haitian HIV-1 isolates than to African isolates. This finding and the presence of a GWGR V3 loop motif in some Brazilian isolates may be important for vaccine development.
Authors: Ana T Dumans; Marcelo A Soares; Danuta Pieniazek; Marcia L Kalish; Veronique De Vroey; Kurt Hertogs; Amilcar Tanuri Journal: Antimicrob Agents Chemother Date: 2002-09 Impact factor: 5.191
Authors: L M Janini; D Pieniazek; J M Peralta; M Schechter; A Tanuri; A C Vicente; N dela Torre; N J Pieniazek; C C Luo; M L Kalish; G Schochetman; M A Rayfield Journal: Virus Genes Date: 1996 Impact factor: 2.332
Authors: J P Moore; A Trkola; B Korber; L J Boots; J A Kessler; F E McCutchan; J Mascola; D D Ho; J Robinson; A J Conley Journal: J Virol Date: 1995-01 Impact factor: 5.103
Authors: A Tanuri; A C Vicente; K Otsuki; C A Ramos; O C Ferreira; M Schechter; L M Janini; D Pieniazek; M A Rayfield Journal: Antimicrob Agents Chemother Date: 1999-02 Impact factor: 5.191
Authors: R Brindeiro; B Vanderborght; E Caride; L Correa; R M Oravec; O Berro; L Stuyver; A Tanuri Journal: Antimicrob Agents Chemother Date: 1999-07 Impact factor: 5.191
Authors: A Trkola; A B Pomales; H Yuan; B Korber; P J Maddon; G P Allaway; H Katinger; C F Barbas; D R Burton; D D Ho Journal: J Virol Date: 1995-11 Impact factor: 5.103
Authors: R Q Warren; M T Wong; G P Melcher; S P Blatt; P Cahn; H Perez; I Zapiola; M B Bouzas; G Muchinik; S A Anderson Journal: J Clin Microbiol Date: 1995-02 Impact factor: 5.948