Literature DB >> 8214598

Detection of ascorbate peroxidase activity in native gels by inhibition of the ascorbate-dependent reduction of nitroblue tetrazolium.

R Mittler1, B A Zilinskas.   

Abstract

A method for the detection of ascorbate peroxidase activity in native electrophoretic gels is described. The assay is based on the ability of ascorbate peroxidase to prevent the ascorbate-dependent reduction of nitroblue tetrazolium in the presence of H2O2. The method was found to be both sensitive (detection of less than 0.01 units of ascorbate peroxidase activity) and specific for ascorbate peroxidase activity. The application of the method for the detection of ascorbate peroxidase activity in protein extracts from several plant sources was investigated by comparing staining for activities of ascorbate peroxidase, horseradish peroxidase, and ascorbate oxidase and by immunodetection of ascorbate peroxidase in these extracts.

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Year:  1993        PMID： 8214598     DOI： 10.1006/abio.1993.1366

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  56 in total

1.  Enzymes that scavenge reactive oxygen species are down-regulated prior to gibberellic acid-induced programmed cell death in barley aleurone.

Authors:  A Fath; P C Bethke; R L Jones
Journal:  Plant Physiol       Date:  2001-05       Impact factor: 8.340

2.  S-nitrosylation positively regulates ascorbate peroxidase activity during plant stress responses.

Authors:  Huanjie Yang; Jinye Mu; Lichao Chen; Jian Feng; Jiliang Hu; Lei Li; Jian-Min Zhou; Jianru Zuo
Journal:  Plant Physiol       Date:  2015-02-09       Impact factor: 8.340

3.  Light and excess manganese . Implications for oxidative stress in common bean

Authors: 
Journal:  Plant Physiol       Date:  1998-10       Impact factor: 8.340

4.  An O-acetylserine(thiol)lyase homolog with L-cysteine desulfhydrase activity regulates cysteine homeostasis in Arabidopsis.

Authors:  Consolación Alvarez; Leticia Calo; Luis C Romero; Irene García; Cecilia Gotor
Journal:  Plant Physiol       Date:  2009-12-02       Impact factor: 8.340

5.  Differential Localization of Antioxidants in Maize Leaves.

Authors:  A. G. Doulis; N. Debian; A. H. Kingston-Smith; C. H. Foyer
Journal:  Plant Physiol       Date:  1997-07       Impact factor: 8.340

6.  Responses of Antioxidants to Paraquat in Pea Leaves (Relationships to Resistance).

Authors:  J. L. Donahue; C. M. Okpodu; C. L. Cramer; E. A. Grabau; R. G. Alscher
Journal:  Plant Physiol       Date:  1997-01       Impact factor: 8.340

7.  Overproduction of Ascorbate Peroxidase in the Tobacco Chloroplast Does Not Provide Protection against Ozone.

Authors:  G. Torsethaugen; L. H. Pitcher; B. A. Zilinskas; E. J. Pell
Journal:  Plant Physiol       Date:  1997-06       Impact factor: 8.340

8.  Amelioration of Ozone-Induced Oxidative Damage in Wheat Plants Grown under High Carbon Dioxide (Role of Antioxidant Enzymes).

Authors:  M. V. Rao; B. A. Hale; D. P. Ormrod
Journal:  Plant Physiol       Date:  1995-10       Impact factor: 8.340

9.  Changes in Isozyme Profiles of Catalase, Peroxidase, and Glutathione Reductase during Acclimation to Chilling in Mesocotyls of Maize Seedlings.

Authors:  M. D. Anderson; T. K. Prasad; C. R. Stewart
Journal:  Plant Physiol       Date:  1995-12       Impact factor: 8.340

10.  Evidence for the Presence of the Ascorbate-Glutathione Cycle in Mitochondria and Peroxisomes of Pea Leaves.

Authors:  A. Jimenez; J. A. Hernandez; L. A. Del Rio; F. Sevilla
Journal:  Plant Physiol       Date:  1997-05       Impact factor: 8.340
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