Identification of four genes involved in the lysogenic pathway of the Salmonella newington bacterial virus epsilon 34.

A structure/function study has been initiated for the epsilon 34 bacteriophage proteins involved in lysogeny in Salmonella newington. Hydroxylamine and nitrosoguanidine mutagenesis of a wild type epsilon 34 phage was used to generate clear plaque variants. Complementation analysis was used to define four genes involved in the phage lysogenic pathway. A relative mapping order has been established. In addition, a virulent mutant, epsilon 34vir82, which defines a repressor binding site has been isolated.