| Literature DB >> 8195101 |
C Llanes1, P Gabant, M Couturier, Y Michel-Briand.
Abstract
The Inc A/C plasmids, like Inc P and Inc Q plasmids, have a broad host range. However, their maintenance functions remain to be studied. An autoreplicative region of 2.79 kb named RepA/C, able to replicate both in the family Enterobacteriaceae and in Pseudomonas spp., was isolated and sequenced. The stability, copy number, and incompatibility expression of this replicon were determined. RepA/C and a nonautoreplicative fragment of 16 kb of this replicon were used as probes and showed specific hybridizations with the Inc P3-A/C plasmids from Pseudomonas spp. and members of the Enterobacteriaceae. These probes could be used as tools for identification of the plasmids of this epidemiologically important Inc group.Entities:
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Year: 1994 PMID: 8195101 PMCID: PMC205517 DOI: 10.1128/jb.176.11.3403-3407.1994
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490