Literature DB >> 8185920

Large-scale supercoiled plasmid preparation by acidic phenol extraction.

Z Wang1, T G Rossman.   

Abstract

A novel method for large-scale plasmid preparation is described. Crude extracts are subjected to acidic phenol extraction to remove any contaminants present in the aqueous phase. The supercoiled plasmid DNA, which preferentially remains in the organic phase and inter-phase, is extracted back into the aqueous phase with 1.5 M TRIZMA base, from which it is precipitated. The resultant plasmid DNA is highly pure and satisfactory for any subsequent procedures. The method is extremely economical and takes only 3-4 h.

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Year:  1994        PMID： 8185920

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  13 in total

1.  Antimicrobial resistance genes associated with Salmonella enterica serovar newport isolates from food animals.

Authors:  Aaron M Lynne; Bobbie S Rhodes-Clark; Kimberly Bliven; Shaohua Zhao; Steven L Foley
Journal:  Antimicrob Agents Chemother       Date:  2007-10-29       Impact factor: 5.191

2.  Efficient repair of all types of single-base mismatches in recombination intermediates in Chinese hamster ovary cells. Competition between long-patch and G-T glycosylase-mediated repair of G-T mismatches.

Authors:  C A Bill; W A Duran; N R Miselis; J A Nickoloff
Journal:  Genetics       Date:  1998-08       Impact factor: 4.562

3.  Mismatch repair by efficient nick-directed, and less efficient mismatch-specific, mechanisms in homologous recombination intermediates in Chinese hamster ovary cells.

Authors:  E M Miller; H L Hough; J W Cho; J A Nickoloff
Journal:  Genetics       Date:  1997-10       Impact factor: 4.562

4.  Biased short tract repair of palindromic loop mismatches in mammalian cells.

Authors:  D G Taghian; H Hough; J A Nickoloff
Journal:  Genetics       Date:  1998-03       Impact factor: 4.562

5.  DNA sequence and comparative genomics of pAPEC-O2-R, an avian pathogenic Escherichia coli transmissible R plasmid.

Authors:  Timothy J Johnson; Kylie E Siek; Sara J Johnson; Lisa K Nolan
Journal:  Antimicrob Agents Chemother       Date:  2005-11       Impact factor: 5.191

6.  Complete DNA sequence of a ColBM plasmid from avian pathogenic Escherichia coli suggests that it evolved from closely related ColV virulence plasmids.

Authors:  Timothy J Johnson; Sara J Johnson; Lisa K Nolan
Journal:  J Bacteriol       Date:  2006-08       Impact factor: 3.490

7.  Topoisomerase II poisoning by indazole and imidazole complexes of ruthenium.

Authors:  Y N Vashisht Gopal; A K Kondapi
Journal:  J Biosci       Date:  2001-06       Impact factor: 1.826

8.  Acquisition of avian pathogenic Escherichia coli plasmids by a commensal E. coli isolate enhances its abilities to kill chicken embryos, grow in human urine, and colonize the murine kidney.

Authors:  Jerod A Skyberg; Timothy J Johnson; James R Johnson; Connie Clabots; Catherine M Logue; Lisa K Nolan
Journal:  Infect Immun       Date:  2006-09-05       Impact factor: 3.441

9.  DNA sequence of a ColV plasmid and prevalence of selected plasmid-encoded virulence genes among avian Escherichia coli strains.

Authors:  Timothy J Johnson; Kylie E Siek; Sara J Johnson; Lisa K Nolan
Journal:  J Bacteriol       Date:  2006-01       Impact factor: 3.490

10.  Characterization of Salmonella enterica serovar Heidelberg from turkey-associated sources.

Authors:  Pravin Kaldhone; Rajesh Nayak; Aaron M Lynne; Donna E David; Patrick F McDermott; Catherine M Logue; Steven L Foley
Journal:  Appl Environ Microbiol       Date:  2008-06-27       Impact factor: 4.792
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