Literature DB >> 8169231

Use of lac gene fusions in the analysis of Acholeplasma upstream gene regulatory sequences.

K L Knudtson1, F C Minion.   

Abstract

The objective of this study was to develop a means of identifying and analyzing mycoplasma gene regulatory elements. Analysis of Acholeplasma oculi ISM1499 transcriptional sequences was accomplished using a promoterless lacZ reporter gene and an integrative vector strategy. Seven promoter-containing fragments were identified, levels of beta-galactosidase were determined, and transcriptional start sites were mapped.

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Year:  1994        PMID： 8169231      PMCID： PMC205422          DOI： 10.1128/jb.176.9.2763-2766.1994

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  11 in total

1.  Development of a cloning system in Mycoplasma pulmonis.

Authors:  G G Mahairas; C Jian; F C Minion
Journal:  Gene       Date:  1990-09-01       Impact factor: 3.688

2.  Sequences upstream of the-35 hexamer of rrnB P1 affect promoter strength and upstream activation.

Authors:  C A Josaitis; T Gaal; W Ross; R L Gourse
Journal:  Biochim Biophys Acta       Date:  1990-08-27

3.  Genetic exchange of transposon and integrative plasmid markers in Mycoplasma pulmonis.

Authors:  G G Mahairas; J A Cao; F C Minion
Journal:  J Bacteriol       Date:  1990-05       Impact factor: 3.490

Review 4.  The mycoplasma genome. Part 1.

Authors:  C Christiansen
Journal:  Microbiol Sci       Date:  1987-06

5.  Analysis of the nucleotide sequence of the P1 operon of Mycoplasma pneumoniae.

Authors:  J M Inamine; S Loechel; P C Hu
Journal:  Gene       Date:  1988-12-15       Impact factor: 3.688

Review 6.  Compilation and analysis of Escherichia coli promoter DNA sequences.

Authors:  D K Hawley; W R McClure
Journal:  Nucleic Acids Res       Date:  1983-04-25       Impact factor: 16.971

7.  Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast.

Authors:  M J Casadaban; A Martinez-Arias; S K Shapira; J Chou
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

Review 8.  Molecular biology and genetics of mycoplasmas (Mollicutes).

Authors:  S Razin
Journal:  Microbiol Rev       Date:  1985-12

9.  Cloning of Streptococcus pneumoniae DNA fragments in Escherichia coli requires vectors protected by strong transcriptional terminators.

Authors:  J D Chen; D A Morrison
Journal:  Gene       Date:  1987       Impact factor: 3.688

10.  Transformation of Mycoplasma pulmonis: demonstration of homologous recombination, introduction of cloned genes, and preliminary description of an integrating shuttle system.

Authors:  G G Mahairas; F C Minion
Journal:  J Bacteriol       Date:  1989-04       Impact factor: 3.490
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  3 in total

1.  Probing in vivo promoter activities in Mycoplasma pneumoniae: a system for generation of single-copy reporter constructs.

Authors:  Sven Halbedel; Jörg Stülke
Journal:  Appl Environ Microbiol       Date:  2006-02       Impact factor: 4.792

2.  Cloning of mnuA, a membrane nuclease gene of Mycoplasma pulmonis, and analysis of its expression in Escherichia coli.

Authors:  K J Jarvill-Taylor; C VanDyk; F C Minion
Journal:  J Bacteriol       Date:  1999-03       Impact factor: 3.490

3.  A novel transposon construct expressing PhoA with potential for studying protein expression and translocation in Mycoplasma gallisepticum.

Authors:  Indu S Panicker; Anna Kanci; Chien-Ju Chiu; Paul D Veith; Michelle D Glew; Glenn F Browning; Philip F Markham
Journal:  BMC Microbiol       Date:  2012-07-08       Impact factor: 3.605
  3 in total

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