Literature DB >> 8161168

A method to detect enteroviruses in sewage sludge-amended soil using the PCR.

T M Straub1, I L Pepper, M Abbaszadegan, C P Gerba.   

Abstract

PCR detection of seeded poliovirus type 1 in sludge-amended soil was made possible by utilizing Sephadex G-50 and Chelex-100 resins to remove compounds present in sludge-amended soil that may inhibit PCR. With this method, enteroviruses indigenous to an anerobically digested sludge were detected by PCR in 10 different soils amended with this sludge.

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Year:  1994        PMID： 8161168      PMCID： PMC201425          DOI： 10.1128/aem.60.3.1014-1017.1994

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  6 in total

1.  Presence of human immunodeficiency virus nucleic acids in wastewater and their detection by polymerase chain reaction.

Authors:  S A Ansari; S R Farrah; G R Chaudhry
Journal:  Appl Environ Microbiol       Date:  1992-12       Impact factor: 4.792

2.  Detection of naturally occurring enteroviruses in waters by reverse transcription, polymerase chain reaction, and hybridization.

Authors:  H Kopecka; S Dubrou; J Prevot; J Marechal; J M López-Pila
Journal:  Appl Environ Microbiol       Date:  1993-04       Impact factor: 4.792

3.  Inactivation of enteric viruses in wastewater sludge through dewatering by evaporation.

Authors:  R L Ward; C S Ashley
Journal:  Appl Environ Microbiol       Date:  1977-11       Impact factor: 4.792

4.  Enterovirus inactivation in soil.

Authors:  J G Yeager; R T O'Brien
Journal:  Appl Environ Microbiol       Date:  1979-10       Impact factor: 4.792

5.  Detection of enteroviruses in groundwater with the polymerase chain reaction.

Authors:  M Abbaszadegan; M S Huber; C P Gerba; I L Pepper
Journal:  Appl Environ Microbiol       Date:  1993-05       Impact factor: 4.792

6.  Detection of Escherichia coli in sewage and sludge by polymerase chain reaction.

Authors:  Y L Tsai; C J Palmer; L R Sangermano
Journal:  Appl Environ Microbiol       Date:  1993-02       Impact factor: 4.792
  6 in total
  14 in total

1.  A method to detect low levels of enteric viruses in contaminated oysters.

Authors:  Y C Shieh; K R Calci; R S Baric
Journal:  Appl Environ Microbiol       Date:  1999-11       Impact factor: 4.792

2.  Comparison of PCR and cell culture for detection of enteroviruses in sludge-amended field soils and determination of their transport.

Authors:  T M Straub; I L Pepper; C P Gerba
Journal:  Appl Environ Microbiol       Date:  1995-05       Impact factor: 4.792

Review 3.  Inhibition and facilitation of nucleic acid amplification.

Authors:  I G Wilson
Journal:  Appl Environ Microbiol       Date:  1997-10       Impact factor: 4.792

4.  Occurrence and persistence of bacterial pathogens and indicator organisms in beach sand along the California coast.

Authors:  Kevan M Yamahara; Lauren M Sassoubre; Kelly D Goodwin; Alexandria B Boehm
Journal:  Appl Environ Microbiol       Date:  2012-01-13       Impact factor: 4.792

5.  Genotyping of rotaviruses isolated from sewage.

Authors:  R Gajardo; N Bouchriti; R M Pintó; A Bosch
Journal:  Appl Environ Microbiol       Date:  1995-09       Impact factor: 4.792

6.  Detection of methanotrophic bacteria in environmental samples with the PCR.

Authors:  I R McDonald; E M Kenna; J C Murrell
Journal:  Appl Environ Microbiol       Date:  1995-01       Impact factor: 4.792

7.  Detection of hepatitis A virus, rotavirus, and enterovirus in naturally contaminated shellfish and sediment by reverse transcription-seminested PCR.

Authors:  F Le Guyader; E Dubois; D Menard; M Pommepuy
Journal:  Appl Environ Microbiol       Date:  1994-10       Impact factor: 4.792

8.  Development and evaluation of methods to detect nucleopolyhedroviruses in larvae of the Douglas-fir tussock moth, Orgyia pseudotsugata (McDunnough).

Authors:  Christine M Thorne; Imre S Otvos; Nicholas Conder; David B Levin
Journal:  Appl Environ Microbiol       Date:  2006-12-22       Impact factor: 4.792

9.  Bacterial community structures of phosphate-removing and non-phosphate-removing activated sludges from sequencing batch reactors.

Authors:  P L Bond; P Hugenholtz; J Keller; L L Blackall
Journal:  Appl Environ Microbiol       Date:  1995-05       Impact factor: 4.792

10.  Cell culture and PCR determination of poliovirus inactivation by disinfectants.

Authors:  J F Ma; T M Straub; I L Pepper; C P Gerba
Journal:  Appl Environ Microbiol       Date:  1994-11       Impact factor: 4.792
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