Literature DB >> 3443622

New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues.

E Hochuli1, H Döbeli, A Schacher.   

Abstract

A novel nitrilotriacetic acid adsorbent has been prepared for metal chelate affinity chromatography. The new resin is a quadridentate chelate former and specially suitable for metal ions with coordination numbers of six, since two valencies remain for the reversible binding of biopolymers. It has been found to chelate Cu2+ and Ni2+ strongly and to be superior to the known iminodiacetic acid adsorbent. Charged with Ni2+, it was evaluated for the ability to bind peptides and proteins containing neighbouring histidine residues. The remarkable specificity found makes it an attractive addition to the range of adsorbents for metal chelate affinity chromatography.

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Year:  1987        PMID： 3443622     DOI： 10.1016/s0021-9673(00)93969-4

Source DB:  PubMed          Journal:  J Chromatogr


  178 in total

1.  Cloning of an intracellular Poly[D(-)-3-Hydroxybutyrate] depolymerase gene from Ralstonia eutropha H16 and characterization of the gene product.

Authors:  H Saegusa; M Shiraki; C Kanai; T Saito
Journal:  J Bacteriol       Date:  2001-01       Impact factor: 3.490

2.  Purification of proteins using polyhistidine affinity tags.

Authors:  J A Bornhorst; J J Falke
Journal:  Methods Enzymol       Date:  2000       Impact factor: 1.600

3.  Construction of a fluorescent biosensor family.

Authors:  Robert M de Lorimier; J Jeff Smith; Mary A Dwyer; Loren L Looger; Kevin M Sali; Chad D Paavola; Shahir S Rizk; Shamil Sadigov; David W Conrad; Leslie Loew; Homme W Hellinga
Journal:  Protein Sci       Date:  2002-11       Impact factor: 6.725

Review 4.  Inorganic Complexes and Metal-Based Nanomaterials for Infectious Disease Diagnostics.

Authors:  Christine F Markwalter; Andrew G Kantor; Carson P Moore; Kelly A Richardson; David W Wright
Journal:  Chem Rev       Date:  2018-12-04       Impact factor: 60.622

5.  Chicken MAR-binding protein ARBP is homologous to rat methyl-CpG-binding protein MeCP2.

Authors:  J M Weitzel; H Buhrmester; W H Strätling
Journal:  Mol Cell Biol       Date:  1997-09       Impact factor: 4.272

6.  Imaging whole Escherichia coli bacteria by using single-particle x-ray diffraction.

Authors:  Jianwei Miao; Keith O Hodgson; Tetsuya Ishikawa; Carolyn A Larabell; Mark A LeGros; Yoshinori Nishino
Journal:  Proc Natl Acad Sci U S A       Date:  2002-12-23       Impact factor: 11.205

7.  Probing the substrate specificity of Escherichia coli RNase E using a novel oligonucleotide-based assay.

Authors:  Vladimir R Kaberdin
Journal:  Nucleic Acids Res       Date:  2003-08-15       Impact factor: 16.971

8.  The PERIANTHIA gene encodes a bZIP protein involved in the determination of floral organ number in Arabidopsis thaliana.

Authors:  C F Chuang; M P Running; R W Williams; E M Meyerowitz
Journal:  Genes Dev       Date:  1999-02-01       Impact factor: 11.361

9.  A novel epitope on the C-terminus of SmD1 is recognized by the majority of sera from patients with systemic lupus erythematosus.

Authors:  G Riemekasten; J Marell; G Trebeljahr; R Klein; G Hausdorf; T Häupl; J Schneider-Mergener; G R Burmester; F Hiepe
Journal:  J Clin Invest       Date:  1998-08-15       Impact factor: 14.808

10.  Mycobacterium tuberculosis expresses two chaperonin-60 homologs.

Authors:  T H Kong; A R Coates; P D Butcher; C J Hickman; T M Shinnick
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-01       Impact factor: 11.205
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