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Literature DB >> 8151292

Characterization of the herpes simplex virus type 1 strain 17+ neurovirulence gene RL1 and its expression in a bacterial system.

E A McKie¹, R G Hope, S M Brown, A R MacLean.

Abstract

The DNA sequence of herpes simplex virus type 1 (HSV-1) strain 17+ in the region coding for the polypeptide ICP34.5 predicts a protein of 248 amino acids with a proposed M(r) of 26,158. The entire RL1 open reading frame was cloned into the expression vector pET8c to enable over-expression of ICP34.5 in Escherichia coli. The expressed protein was partially purified and used as an immunogen to produce a polyclonal antiserum in rabbits. Construction of an ICP34.5 null mutant (1771), demonstrated that the predicted open reading frame for ICP34.5 in strain 17+ is correct and confirmed that HSV-1 strain 17+ ICP34.5 specifically determines neurovirulence. The specificity of the anti-serum directed against the E. coli-expressed ICP34.5 was defined by Western blotting of wild-type and RL1-negative infected cell extracts.

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Year: 1994 PMID： 8151292 DOI： 10.1099/0022-1317-75-4-733

Source DB: PubMed Journal: J Gen Virol ISSN： 0022-1317 Impact factor: 3.891

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Cited

11 in total

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2. Activation of NF-κB in CD8+ dendritic cells Ex Vivo by the γ134.5 null mutant correlates with immunity against herpes simplex virus 1.

Authors: Huali Jin; Yijie Ma; Zhipeng Yan; Bellur S Prabhakar; Bin He
Journal: J Virol Date: 2011-11-09 Impact factor: 5.103

3. Signals that dictate nuclear, nucleolar, and cytoplasmic shuttling of the gamma(1)34.5 protein of herpes simplex virus type 1.

Authors: Guofeng Cheng; Marie-Elena Brett; Bin He
Journal: J Virol Date: 2002-09 Impact factor: 5.103

4. A virus with a mutation in the ICP4-binding site in the L/ST promoter of herpes simplex virus type 1, but not a virus with a mutation in open reading frame P, exhibits cell-type-specific expression of gamma(1)34.5 transcripts and latency-associated transcripts.

Authors: L Y Lee; P A Schaffer
Journal: J Virol Date: 1998-05 Impact factor: 5.103

5. The herpes simplex virus virulence factor ICP34.5 and the cellular protein MyD116 complex with proliferating cell nuclear antigen through the 63-amino-acid domain conserved in ICP34.5, MyD116, and GADD34.

Authors: S M Brown; A R MacLean; E A McKie; J Harland
Journal: J Virol Date: 1997-12 Impact factor: 5.103

6. Association of a M(r) 90,000 phosphoprotein with protein kinase PKR in cells exhibiting enhanced phosphorylation of translation initiation factor eIF-2 alpha and premature shutoff of protein synthesis after infection with gamma 134.5- mutants of herpes simplex virus 1.

Authors: J Chou; J J Chen; M Gross; B Roizman
Journal: Proc Natl Acad Sci U S A Date: 1995-11-07 Impact factor: 11.205

10. The herpes simplex virus (HSV) protein ICP34.5 is a virion component that forms a DNA-binding complex with proliferating cell nuclear antigen and HSV replication proteins.

Authors: June Harland; Paul Dunn; Euan Cameron; Joe Conner; S Moira Brown
Journal: J Neurovirol Date: 2003-08 Impact factor: 2.643