Literature DB >> 8150969

Purification of Chlamydia trachomatis strains in mixed infection by monoclonal antibody neutralization.

M F Lampe1, W E Stamm.   

Abstract

A Chlamydia trachomatis D* serovariant strain was found to be mixed with an F serovar strain in a clinical specimen. By using a monoclonal antibody which neutralizes serovar F infectivity in hamster kidney cells, the D* variant strain was enriched until it could be cloned by limiting dilution. This newly described neutralization enrichment procedure can be used to purify a C. trachomatis serovar present in small numbers in a mixed culture or, potentially, to identify nonneutralizable mutants.

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Year:  1994        PMID： 8150969      PMCID： PMC263070          DOI： 10.1128/jcm.32.2.533-535.1994

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  9 in total

Review 1.  Pelvic inflammatory disease: bacteriology and sequelae.

Authors:  L Weström
Journal:  Contraception       Date:  1987-07       Impact factor: 3.375

2.  Nucleotide sequence of the variable domains within the major outer membrane protein gene from serovariants of Chlamydia trachomatis.

Authors:  M F Lampe; R J Suchland; W E Stamm
Journal:  Infect Immun       Date:  1993-01       Impact factor: 3.441

3.  Screening for chlamydial infections in women attending family planning clinics.

Authors:  J Schachter; E Stoner; J Moncada
Journal:  West J Med       Date:  1983-03

4.  Diversity of Chlamydia trachomatis major outer membrane protein genes.

Authors:  R S Stephens; R Sanchez-Pescador; E A Wagar; C Inouye; M S Urdea
Journal:  J Bacteriol       Date:  1987-09       Impact factor: 3.490

5.  Simplified microtiter cell culture method for rapid immunotyping of Chlamydia trachomatis.

Authors:  R J Suchland; W E Stamm
Journal:  J Clin Microbiol       Date:  1991-07       Impact factor: 5.948

6.  Effect of treatment regimens for Neisseria gonorrhoeae on simultaneous infection with Chlamydia trachomatis.

Authors:  W E Stamm; M E Guinan; C Johnson; T Starcher; K K Holmes; W M McCormack
Journal:  N Engl J Med       Date:  1984-03-01       Impact factor: 91.245

7.  Chlamydia trachomatis infections in the United States. What are they costing us?

Authors:  A E Washington; R E Johnson; L L Sanders
Journal:  JAMA       Date:  1987-04-17       Impact factor: 56.272

8.  Detection of Chlamydia trachomatis inclusions in Mccoy cell cultures with fluorescein-conjugated monoclonal antibodies.

Authors:  W E Stamm; M Tam; M Koester; L Cles
Journal:  J Clin Microbiol       Date:  1983-04       Impact factor: 5.948

9.  Incidence, trends, and risks of ectopic pregnancy in a population of women.

Authors:  L Weström; L P Bengtsson; P A Mårdh
Journal:  Br Med J (Clin Res Ed)       Date:  1981-01-03
  9 in total

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