Literature DB >> 8150714

Application of the random amplified polymorphic DNA using the polymerase chain reaction for efficient elimination of duplicate strains in microbial screening. II. Actinomycetes.

Y Anzai1, T Okuda, J Watanabe.   

Abstract

We evaluated the random amplified polymorphic DNA (RAPD) method using Streptomyces lavendulae and Streptomyces virginiae strains to eliminate duplicate actinomycete strains in our microbial screening program. The RAPD data were compared with phenotypic characteristics, DNA relatedness, HPLC analysis of metabolites and low-frequency restriction fragment analysis by pulsed-field gel electrophoresis. These results were consistent with each other. Therefore, we conclude that RAPD is a simple, efficient, and reliable method for the selection of actinomycete strains.

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Year:  1994        PMID： 8150714     DOI： 10.7164/antibiotics.47.183

Source DB:  PubMed          Journal:  J Antibiot (Tokyo)        ISSN： 0021-8820            Impact factor:   2.649


  5 in total

1.  Dereplication of Streptomyces soil isolates and detection of specific biosynthetic genes using an automated ribotyping instrument.

Authors:  F V Ritacco; B Haltli; J E Janso; M Greenstein; V S Bernan
Journal:  J Ind Microbiol Biotechnol       Date:  2003-04-05       Impact factor: 3.346

2.  Use of polymerase chain reaction (PCR) fingerprinting to differentiate bacteria for microbial products screening.

Authors:  C F Hirsch; J M Sigmund
Journal:  J Ind Microbiol       Date:  1995-08

3.  Differentiation of Micromonospora isolates from a coastal sediment in Wales on the basis of Fourier transform infrared spectroscopy, 16S rRNA sequence analysis, and the amplified fragment length polymorphism technique.

Authors:  Hongjuan Zhao; Yankuba Kassama; Michael Young; Douglas B Kell; Royston Goodacre
Journal:  Appl Environ Microbiol       Date:  2004-11       Impact factor: 4.792

4.  Molecular and phenotypic comparison of phaeochromycin-producing strains of Streptomyces phaeochromogenes and Streptomyces ederensis.

Authors:  Frank V Ritacco; Douglas E Eveleigh
Journal:  J Ind Microbiol Biotechnol       Date:  2008-05-17       Impact factor: 3.346

5.  Scaling up a virginiamycin production by a high-yield Streptomyces virginiae VKM Ac-2738D strain using adsorbing resin addition and fed-batch fermentation under controlled conditions.

Authors:  Vakhtang Dzhavakhiya; Vyacheslav Savushkin; Alexander Ovchinnikov; Vladislav Glagolev; Veronika Savelyeva; Evgeniya Popova; Nikita Novak; Elena Glagoleva
Journal:  3 Biotech       Date:  2016-11-12       Impact factor: 2.406
  5 in total

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