Literature DB >> 8141761

Increased salt concentration reversibly destabilizes p53 quaternary structure and sequence-specific DNA binding.

S Butcher1, P Hainaut, J Milner.   

Abstract

Growth suppression by p53 correlates with sequence-specific DNA binding and is determined by tertiary and quaternary protein structures. Exposure to 300 mM NaCl did not affect p53 tertiary structure, but dissociated high-molecular-mass complexes with concomitant loss of specific DNA binding. Both effects were reversible. We conclude that high salt can reversibly destabilize the quaternary structure of p53 that is most efficient for sequence-specific DNA binding.

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Year:  1994        PMID： 8141761      PMCID： PMC1137888          DOI： 10.1042/bj2980513

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  25 in total

1.  Protein-protein interactions in high molecular weight forms of the transformation-related phosphoprotein p53.

Authors:  S Kraiss; A Lorenz; M Montenarh
Journal:  Biochim Biophys Acta       Date:  1992-02-13

2.  Cotranslation of activated mutant p53 with wild type drives the wild-type p53 protein into the mutant conformation.

Authors:  J Milner; E A Medcalf
Journal:  Cell       Date:  1991-05-31       Impact factor: 41.582

3.  A transcriptionally active DNA-binding site for human p53 protein complexes.

Authors:  W D Funk; D T Pak; R H Karas; W E Wright; J W Shay
Journal:  Mol Cell Biol       Date:  1992-06       Impact factor: 4.272

4.  Tumor suppressor p53: analysis of wild-type and mutant p53 complexes.

Authors:  J Milner; E A Medcalf; A C Cook
Journal:  Mol Cell Biol       Date:  1991-01       Impact factor: 4.272

Review 5.  A conformation hypothesis for the suppressor and promoter functions of p53 in cell growth control and in cancer.

Authors:  J Milner
Journal:  Proc Biol Sci       Date:  1991-08-22       Impact factor: 5.349

6.  Analysis of p53 quaternary structure in relation to sequence-specific DNA binding.

Authors:  P Hainaut; A Hall; J Milner
Journal:  Oncogene       Date:  1994-01       Impact factor: 9.867

7.  Different forms of p53 detected by monoclonal antibodies in non-dividing and dividing lymphocytes.

Authors:  J Milner
Journal:  Nature       Date:  1984 Jul 12-18       Impact factor: 49.962

8.  Temperature-dependent switching between "wild-type" and "mutant" forms of p53-Val135.

Authors:  J Milner; E A Medcalf
Journal:  J Mol Biol       Date:  1990-12-05       Impact factor: 5.469

9.  The p53 tumour suppressor protein is phosphorylated at serine 389 by casein kinase II.

Authors:  D W Meek; S Simon; U Kikkawa; W Eckhart
Journal:  EMBO J       Date:  1990-10       Impact factor: 11.598

10.  Analysis of equilibrium and kinetic measurements to determine thermodynamic origins of stability and specificity and mechanism of formation of site-specific complexes between proteins and helical DNA.

Authors:  M T Record; J H Ha; M A Fisher
Journal:  Methods Enzymol       Date:  1991       Impact factor: 1.600
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  3 in total

1.  Redox state of tumor suppressor p53 regulates its sequence-specific DNA binding in DNA-damaged cells by cysteine 277.

Authors:  Jiri Buzek; Leena Latonen; Sari Kurki; Karita Peltonen; Marikki Laiho
Journal:  Nucleic Acids Res       Date:  2002-06-01       Impact factor: 16.971

2.  Differential salt-induced dissociation of the p53 protein complexes with circular and linear plasmid DNA substrates suggest involvement of a sliding mechanism.

Authors:  Peter Šebest; Marie Brázdová; Miroslav Fojta; Hana Pivoňková
Journal:  Int J Mol Sci       Date:  2015-01-30       Impact factor: 5.923

3.  ExoMeg1: a new exonuclease from metagenomic library.

Authors:  Rita C B Silva-Portela; Fabíola M Carvalho; Carolina P M Pereira; Nadja C de Souza-Pinto; Mauro Modesti; Robert P Fuchs; Lucymara F Agnez-Lima
Journal:  Sci Rep       Date:  2016-01-27       Impact factor: 4.379
  3 in total

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