Literature DB >> 8139400

An improved method for the measurement of malondialdehyde in biological samples.

X Y Li1, C K Chow.   

Abstract

An improved method was developed for measuring malondialdehyde (MDA) as its thiobarbituric acid (TBA) complex. Samples were initially incubated with 1% potassium iodide and 0.1% butylated hydroxytoluene at 50 degrees C for 20 min, and then with 0.4% TBA at 60 degrees C for 60 min. The MDA-TBA complex formed was extracted with isobutyl alcohol and measured by high-performance liquid chromatography with fluorescence detection. The improved method allows for a more specific determination of MDA present in biological samples.

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Year:  1994        PMID： 8139400     DOI： 10.1007/bf02537094

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  11 in total

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Journal:  Lipids       Date:  1987-09       Impact factor: 1.880

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Journal:  Methods Enzymol       Date:  1984       Impact factor: 1.600

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Journal:  Methods Enzymol       Date:  1984       Impact factor: 1.600

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Journal:  Methods Enzymol       Date:  1978       Impact factor: 1.600

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Journal:  Free Radic Biol Med       Date:  1991       Impact factor: 7.376

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Authors:  C K Chow
Journal:  Free Radic Biol Med       Date:  1991       Impact factor: 7.376

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Authors:  G M Siu; H H Draper
Journal:  Lipids       Date:  1982-05       Impact factor: 1.880
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9.  Oxidative damage and inflammation in obese diabetic Emirati subjects supplemented with antioxidants and B-vitamins: a randomized placebo-controlled trail.

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