Literature DB >> 8132710

En bloc incorporation of coatomer subunits during the assembly of COP-coated vesicles.

S Hara-Kuge1, O Kuge, L Orci, M Amherdt, M Ravazzola, F T Wieland, J E Rothman.   

Abstract

The cDNA encoding epsilon-COP, the 36-kD subunit of coatomer, was cloned from a bovine liver cDNA library and sequenced. Immunoblotting with an anti-epsilon-COP antibody showed that epsilon-COP exists in COP-coated vesicles as well as in the cytosolic coatomer. Using the cloned cDNA, recombinant His6- tagged epsilon-COP was overexpressed in cultured Chinese hamster ovary (CHO) cells, from which metabolically radiolabeled coatomer was purified by taking advantage of the His6 tag. Radiolabeled coatomer was employed to establish that all the subunits of the coatomer enter coated vesicles as an intact unit.

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Year:  1994        PMID： 8132710      PMCID： PMC2119964          DOI： 10.1083/jcb.124.6.883

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  26 in total

1.  Internal sequence analysis of proteins separated on polyacrylamide gels at the submicrogram level: improved methods, applications and gene cloning strategies.

Authors:  P Tempst; A J Link; L R Riviere; M Fleming; C Elicone
Journal:  Electrophoresis       Date:  1990-07       Impact factor: 3.535

2.  Purification of a novel class of coated vesicles mediating biosynthetic protein transport through the Golgi stack.

Authors:  V Malhotra; T Serafini; L Orci; J C Shepherd; J E Rothman
Journal:  Cell       Date:  1989-07-28       Impact factor: 41.582

3.  Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.

Authors:  W I Wood; J Gitschier; L A Lasky; R M Lawn
Journal:  Proc Natl Acad Sci U S A       Date:  1985-03       Impact factor: 11.205

4.  A new type of coated vesicular carrier that appears not to contain clathrin: its possible role in protein transport within the Golgi stack.

Authors:  L Orci; B S Glick; J E Rothman
Journal:  Cell       Date:  1986-07-18       Impact factor: 41.582

5.  Dissection of a single round of vesicular transport: sequential intermediates for intercisternal movement in the Golgi stack.

Authors:  L Orci; V Malhotra; M Amherdt; T Serafini; J E Rothman
Journal:  Cell       Date:  1989-02-10       Impact factor: 41.582

6.  Examination of automated polypeptide sequencing using standard phenyl isothiocyanate reagent and subpicomole high-performance liquid chromatographic analysis.

Authors:  P Tempst; L Riviere
Journal:  Anal Biochem       Date:  1989-12       Impact factor: 3.365

7.  Characterization of protein transport between successive compartments of the Golgi apparatus: asymmetric properties of donor and acceptor activities in a cell-free system.

Authors:  W E Balch; J E Rothman
Journal:  Arch Biochem Biophys       Date:  1985-07       Impact factor: 4.013

8.  Reconstitution of the transport of protein between successive compartments of the Golgi measured by the coupled incorporation of N-acetylglucosamine.

Authors:  W E Balch; W G Dunphy; W A Braell; J E Rothman
Journal:  Cell       Date:  1984-12       Impact factor: 41.582

9.  Intracellular transport of secretory proteins in the pancreatic exocrine cell. I. Role of the peripheral elements of the Golgi complex.

Authors:  J D Jamieson; G E Palade
Journal:  J Cell Biol       Date:  1967-08       Impact factor: 10.539

10.  Intercompartmental transport in the Golgi complex is a dissociative process: facile transfer of membrane protein between two Golgi populations.

Authors:  J E Rothman; R L Miller; L J Urbani
Journal:  J Cell Biol       Date:  1984-07       Impact factor: 10.539
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  49 in total

1.  In situ localization and in vitro induction of plant COPI-coated vesicles.

Authors:  P Pimpl; A Movafeghi; S Coughlan; J Denecke; S Hillmer; D G Robinson
Journal:  Plant Cell       Date:  2000-11       Impact factor: 11.277

2.  Crystal structure of alpha-COP in complex with epsilon-COP provides insight into the architecture of the COPI vesicular coat.

Authors:  Kuo-Chiang Hsia; André Hoelz
Journal:  Proc Natl Acad Sci U S A       Date:  2010-06-03       Impact factor: 11.205

Review 3.  Retrograde vesicle transport in the Golgi.

Authors:  Nathanael P Cottam; Daniel Ungar
Journal:  Protoplasma       Date:  2011-12-12       Impact factor: 3.356

Review 4.  New links between vesicle coats and Rab-mediated vesicle targeting.

Authors:  Cortney G Angers; Alexey J Merz
Journal:  Semin Cell Dev Biol       Date:  2010-07-17       Impact factor: 7.727

Review 5.  p24 family proteins: key players in the regulation of trafficking along the secretory pathway.

Authors:  Noelia Pastor-Cantizano; Juan Carlos Montesinos; César Bernat-Silvestre; María Jesús Marcote; Fernando Aniento
Journal:  Protoplasma       Date:  2015-07-30       Impact factor: 3.356

6.  Differential induction of two p24delta putative cargo receptors upon activation of a prohormone-producing cell.

Authors:  R P Kuiper; H R Waterham; J Rötter; G Bouw; G J Martens
Journal:  Mol Biol Cell       Date:  2000-01       Impact factor: 4.138

Review 7.  COPI-mediated transport.

Authors:  J Béthune; F Wieland; J Moelleken
Journal:  J Membr Biol       Date:  2006-10-14       Impact factor: 1.843

8.  COPI coatomer complex proteins facilitate the translocation of anthrax lethal factor across vesicular membranes in vitro.

Authors:  Alfred G Tamayo; Ajit Bharti; Carolina Trujillo; Robert Harrison; John R Murphy
Journal:  Proc Natl Acad Sci U S A       Date:  2008-03-20       Impact factor: 11.205

Review 9.  The evolving understanding of COPI vesicle formation.

Authors:  Victor W Hsu; Stella Y Lee; Jia-Shu Yang
Journal:  Nat Rev Mol Cell Biol       Date:  2009-03-18       Impact factor: 94.444

Review 10.  Retrograde traffic from the Golgi to the endoplasmic reticulum.

Authors:  Anne Spang
Journal:  Cold Spring Harb Perspect Biol       Date:  2013-06-01       Impact factor: 10.005
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