Literature DB >> 8132208

Isolation, sequence and expression of a cDNA encoding the alpha-chain of the feline CD8.

M R Pecoraro1, Y Kawaguchi, T Miyazawa, J Norimine, K Maeda, T Toyosaki, Y Tohya, C Kai, T Mikami.   

Abstract

We have cloned, sequenced and expressed a cDNA encoding the alpha-chain of feline CD8. This clone, named FT8-10, has an open reading frame with 720 nucleotides in length encoding a protein with 239 amino acid residues. Sequence analysis has revealed that the feline CD8 alpha-chain (CD8 alpha) shares significant homology with human (T8/Leu-2), bovine (BoCD8), rat (MRC OX8) and mouse (Lyt-2) CD8 alpha subunits. Cysteine residues as well as the tyrosine kinase p56lck binding site are well conserved. Besides, no putative N-linked glycosylation site was found. Interestingly, immunofluorescence analysis of COS-7 cells transfected with feline CD8 alpha expression plasmid driven by SR alpha promoter showed that the expressed feline CD8 alpha cross-reacted with an anti-human CD8 alpha monoclonal antibody OKT8, but did not react with an anti-feline monoclonal antibody, FT-2, which is thought to recognize the feline analogue of the human T8/Leu-2 and murine Lyt-2 molecules expressed on cytotoxic/suppressor T cells.

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Year:  1994        PMID： 8132208      PMCID： PMC1422291     

Source DB:  PubMed          Journal:  Immunology        ISSN： 0019-2805            Impact factor:   7.397


  30 in total

1.  A general method applicable to the search for similarities in the amino acid sequence of two proteins.

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Journal:  J Mol Biol       Date:  1970-03       Impact factor: 5.469

2.  The isolation and sequence of the gene encoding T8: a molecule defining functional classes of T lymphocytes.

Authors:  D R Littman; Y Thomas; P J Maddon; L Chess; R Axel
Journal:  Cell       Date:  1985-02       Impact factor: 41.582

Review 3.  T cell subsets and the recognition of MHC class.

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Journal:  Immunol Rev       Date:  1983       Impact factor: 12.988

4.  A comprehensive set of sequence analysis programs for the VAX.

Authors:  J Devereux; P Haeberli; O Smithies
Journal:  Nucleic Acids Res       Date:  1984-01-11       Impact factor: 16.971

5.  The T8 antigen is a multimeric complex of two distinct subunits on human thymocytes but consists of homomultimeric forms on peripheral blood T lymphocytes.

Authors:  P M Snow; C Terhorst
Journal:  J Biol Chem       Date:  1983-12-10       Impact factor: 5.157

6.  T cell antigen receptors and the immunoglobulin supergene family.

Authors:  L Hood; M Kronenberg; T Hunkapiller
Journal:  Cell       Date:  1985-02       Impact factor: 41.582

7.  Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage.

Authors:  E L Reinherz; P C Kung; G Goldstein; R H Levey; S F Schlossman
Journal:  Proc Natl Acad Sci U S A       Date:  1980-03       Impact factor: 11.205

8.  Feline CD4 molecules expressed on feline non-lymphoid cell lines are not enough for productive infection of highly lymphotropic feline immunodeficiency virus isolates.

Authors:  J Norimine; T Miyazawa; Y Kawaguchi; K Tomonaga; Y S Shin; T Toyosaki; M Kohmoto; M Niikura; Y Tohya; T Mikami
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574

9.  The major histocompatibility complex-restricted antigen receptor on T cells. II. Role of the L3T4 product.

Authors:  P Marrack; R Endres; R Shimonkevitz; A Zlotnik; D Dialynas; F Fitch; J Kappler
Journal:  J Exp Med       Date:  1983-10-01       Impact factor: 14.307

10.  The role of L3T4 in recognition of Ia by a cytotoxic, H-2Dd-specific T cell hybridoma.

Authors:  J L Greenstein; J Kappler; P Marrack; S J Burakoff
Journal:  J Exp Med       Date:  1984-04-01       Impact factor: 14.307
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  5 in total

1.  A feline CD2 homologue interacts with human red blood cells.

Authors:  Masayuki Shimojima; Yorihiro Nishimura; Takayuki Miyazawa; Kentaro Kato; Kazuya Nakamura; Yoshihiro Izumiya; Hiroomi Akashi; Yukinobu Tohya
Journal:  Immunology       Date:  2002-03       Impact factor: 7.397

2.  Molecular cloning of the feline CD8 beta-chain.

Authors:  M R Pecoraro; M Shimojima; K Maeda; Y Inoshima; Y Kawaguchi; C Kai; T Mikami
Journal:  Immunology       Date:  1996-09       Impact factor: 7.397

3.  Characterization of the diffuse mucosal associated lymphoid tissue of feline small intestine.

Authors:  P Roccabianca; J C Woo; P F Moore
Journal:  Vet Immunol Immunopathol       Date:  2000-06-30       Impact factor: 2.046

4.  A "possible" involvement of TNF-alpha in apoptosis induction in peripheral blood lymphocytes of cats with feline infectious peritonitis.

Authors:  Tomomi Takano; Tsutomu Hohdatsu; Yoshikiyo Hashida; Yasuhiro Kaneko; Maki Tanabe; Hiroyuki Koyama
Journal:  Vet Microbiol       Date:  2006-09-16       Impact factor: 3.293

Review 5.  Biological characters of bats in relation to natural reservoir of emerging viruses.

Authors:  Tsutomu Omatsu; Shumpei Watanabe; Hiroomi Akashi; Yasuhiro Yoshikawa
Journal:  Comp Immunol Microbiol Infect Dis       Date:  2007-08-15       Impact factor: 2.268
  5 in total

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