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Literature DB >> 8127668

The ribosomal RNA identity elements for ricin and for alpha-sarcin: mutations in the putative CG pair that closes a GAGA tetraloop.

Abstract

alpha-Sarcin is a ribonuclease that cleaves the phosphodiester bond on the 3' side of G4325 in 28S rRNA; ricin A-chain is a RNA N-glycosidase that depurinates the 5' adjacent A4324. These single covalent modifications inactivate the ribosome. An oligoribonucleotide that reproduces the structure of the sarcin/ricin domain in 28S rRNA was synthesized and mutations were constructed in the 5' C and the 3' G that surround a GAGA tetrad that has the sites of toxin action. Covalent modification of the RNA by ricin, but not by alpha-sarcin, requires a Watson-Crick pair to shut off a putative GAGA tetraloop. Either the recognition elements for the two toxins are different despite their catalyzing covalent modification of adjacent nucleotides in 28S rRNA or there are transitions in the conformation of the alpha-sarcin/ricin domain in 28S rRNA and one conformer is recognized by alpha-sarcin and the other by ricin A-chain.

Entities: Chemical

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Year: 1994 PMID： 8127668 PMCID： PMC523583 DOI： 10.1093/nar/22.3.321

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

15 in total

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Authors: I G Wool; A Glück; Y Endo
Journal: Trends Biochem Sci Date: 1992-07 Impact factor: 13.807

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Authors: C R Woese; S Winker; R R Gutell
Journal: Proc Natl Acad Sci U S A Date: 1990-11 Impact factor: 11.205

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Authors: L Montanaro; S Sperti; A Mattioli; G Testoni; F Stirpe
Journal: Biochem J Date: 1975-01 Impact factor: 3.857

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Authors: Y Endo; K Tsurugi
Journal: J Biol Chem Date: 1987-06-15 Impact factor: 5.157

5. Interaction of elongation factors EF-G and EF-Tu with a conserved loop in 23S RNA.

Authors: D Moazed; J M Robertson; H F Noller
Journal: Nature Date: 1988-07-28 Impact factor: 49.962

6. Evidence that the G2661 region of 23S rRNA is located at the ribosomal binding sites of both elongation factors.

Authors: T P Hausner; J Atmadja; K H Nierhaus
Journal: Biochimie Date: 1987-09 Impact factor: 4.079

7. The cytotoxins alpha-sarcin and ricin retain their specificity when tested on a synthetic oligoribonucleotide (35-mer) that mimics a region of 28 S ribosomal ribonucleic acid.

Authors: Y Endo; Y L Chan; A Lin; K Tsurugi; I G Wool
Journal: J Biol Chem Date: 1988-06-15 Impact factor: 5.157

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Authors: Y Endo; A Glück; Y L Chan; K Tsurugi; I G Wool
Journal: J Biol Chem Date: 1990-02-05 Impact factor: 5.157

9. The RNA N-glycosidase activity of ricin A-chain. The characteristics of the enzymatic activity of ricin A-chain with ribosomes and with rRNA.

Authors: Y Endo; K Tsurugi
Journal: J Biol Chem Date: 1988-06-25 Impact factor: 5.157

10. Structural and sequence elements important for recognition of Escherichia coli formylmethionine tRNA by methionyl-tRNA transformylase are clustered in the acceptor stem.

Authors: C P Lee; B L Seong; U L RajBhandary
Journal: J Biol Chem Date: 1991-09-25 Impact factor: 5.157

15 in total

1. Translational suppressors and antisuppressors alter the efficiency of the Ty1 programmed translational frameshift.

Authors: C L Burck; Y O Chernoff; R Liu; P J Farabaugh; S W Liebman
Journal: RNA Date: 1999-11 Impact factor: 4.942

2. NMR structure of the noncytotoxic alpha-sarcin mutant Delta(7-22): the importance of the native conformation of peripheral loops for activity.

Authors: Ma Flor García-Mayoral; Lucia García-Ortega; Ma Pilar Lillo; Jorge Santoro; Alvaro Martínez del Pozo; José G Gavilanes; Manuel Rico; Marta Bruix
Journal: Protein Sci Date: 2004-04 Impact factor: 6.725

3. The electrostatic character of the ribosomal surface enables extraordinarily rapid target location by ribotoxins.

Authors: Alexei V Korennykh; Joseph A Piccirilli; Carl C Correll
Journal: Nat Struct Mol Biol Date: 2006-04-09 Impact factor: 15.369

4. The sarcin-ricin loop of 23S rRNA is essential for assembly of the functional core of the 50S ribosomal subunit.

Authors: Laura Lancaster; Nicole J Lambert; Eric J Maklan; Lucas H Horan; Harry F Noller
Journal: RNA Date: 2008-08-28 Impact factor: 4.942

Review 5. Enzymatic transition states, transition-state analogs, dynamics, thermodynamics, and lifetimes.

Authors: Vern L Schramm
Journal: Annu Rev Biochem Date: 2011 Impact factor: 23.643

6. Placement of the alpha-sarcin loop within the 50S subunit: evidence derived using a photolabile oligodeoxynucleotide probe.

Authors: P Muralikrishna; R W Alexander; B S Cooperman
Journal: Nucleic Acids Res Date: 1997-11-15 Impact factor: 16.971