Literature DB >> 8126187

Detection of Candida albicans and other yeasts in blood by PCR.

A R Holmes1, R D Cannon, M G Shepherd, H F Jenkinson.   

Abstract

Primers complementary to the region of genes coding for rRNA in Candida albicans were used in PCRs to detect yeast DNA extracted from blood samples containing various Candida species. One fragment (105 bp) was amplified from all yeasts tested, whereas a second (684 bp) was only amplified when C. albicans DNA was present. The level of sensitivity was 15 +/- 5 (mean +/- standard error) CFU of C. albicans per ml of blood.

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Year:  1994        PMID： 8126187      PMCID： PMC263004          DOI： 10.1128/jcm.32.1.228-231.1994

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  Invasive candida infections--evolution of a fungal pathogen.

Authors:  J E Edwards
Journal:  N Engl J Med       Date:  1991-04-11       Impact factor: 91.245

2.  Isolation and nucleotide sequence of an autonomously replicating sequence (ARS) element functional in Candida albicans and Saccharomyces cerevisiae.

Authors:  R D Cannon; H F Jenkinson; M G Shepherd
Journal:  Mol Gen Genet       Date:  1990-04

3.  Candida albicans- and Candida stellatoidea-specific DNA fragment.

Authors:  J E Cutler; P M Glee; H L Horn
Journal:  J Clin Microbiol       Date:  1988-09       Impact factor: 5.948

4.  Isolation of genomic DNA.

Authors:  B G Herrmann; A M Frischauf
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

Review 5.  Laboratory diagnosis of invasive candidiasis.

Authors:  J M Jones
Journal:  Clin Microbiol Rev       Date:  1990-01       Impact factor: 26.132

6.  Detection of surgical pathogens by in vitro DNA amplification. Part I. Rapid identification of Candida albicans by in vitro amplification of a fungus-specific gene.

Authors:  T G Buchman; M Rossier; W G Merz; P Charache
Journal:  Surgery       Date:  1990-08       Impact factor: 3.982

7.  New centrifugation blood culture device.

Authors:  G L Dorn; K Smith
Journal:  J Clin Microbiol       Date:  1978-01       Impact factor: 5.948

8.  Detection and differentiation of fungi in clinical specimens using polymerase chain reaction (PCR) amplification and restriction enzyme analysis.

Authors:  R L Hopfer; P Walden; S Setterquist; W E Highsmith
Journal:  J Med Vet Mycol       Date:  1993

9.  A Candida albicans dispersed, repeated gene family and its epidemiologic applications.

Authors:  S Scherer; D A Stevens
Journal:  Proc Natl Acad Sci U S A       Date:  1988-03       Impact factor: 11.205

10.  Telomeric and dispersed repeat sequences in Candida yeasts and their use in strain identification.

Authors:  C Sadhu; M J McEachern; E P Rustchenko-Bulgac; J Schmid; D R Soll; J B Hicks
Journal:  J Bacteriol       Date:  1991-01       Impact factor: 3.490
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  32 in total

1.  Identification of various medically important Candida species in clinical specimens by PCR-restriction enzyme analysis.

Authors:  G Morace; M Sanguinetti; B Posteraro; G Lo Cascio; G Fadda
Journal:  J Clin Microbiol       Date:  1997-03       Impact factor: 5.948

2.  Identification of contaminating fungal DNA sequences in Zymolyase.

Authors:  D Rimek; A P Garg; W H Haas; R Kappe
Journal:  J Clin Microbiol       Date:  1999-03       Impact factor: 5.948

3.  PCR monitoring of response to liposomal amphotericin B treatment of systemic candidiasis in neutropenic mice.

Authors:  A J van Deventer; W H Goessens; A van Belkum; E W van Etten; H J van Vliet; H A Verbrugh
Journal:  J Clin Microbiol       Date:  1996-01       Impact factor: 5.948

4.  Rapid identification of Candida species by DNA fingerprinting with PCR.

Authors:  M Thanos; G Schonian; W Meyer; C Schweynoch; Y Graser; T G Mitchell; W Presber; H J Tietz
Journal:  J Clin Microbiol       Date:  1996-03       Impact factor: 5.948

5.  Detection and identification of fungal pathogens in blood by using molecular probes.

Authors:  H Einsele; H Hebart; G Roller; J Löffler; I Rothenhofer; C A Müller; R A Bowden; J van Burik; D Engelhard; L Kanz; U Schumacher
Journal:  J Clin Microbiol       Date:  1997-06       Impact factor: 5.948

6.  Semiquantitative polymerase chain reaction enzyme immunoassay for diagnosis of disseminated candidiasis.

Authors:  J P Burnie; N Golbang; R C Matthews
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1997-05       Impact factor: 3.267

7.  Rapid detection of Candida albicans in clinical samples by DNA amplification of common regions from C. albicans-secreted aspartic proteinase genes.

Authors:  M Flahaut; D Sanglard; M Monod; J Bille; M Rossier
Journal:  J Clin Microbiol       Date:  1998-02       Impact factor: 5.948

8.  PCR detection of DNA specific for Trichosporon species in serum of patients with disseminated trichosporonosis.

Authors:  H Nagai; Y Yamakami; A Hashimoto; I Tokimatsu; M Nasu
Journal:  J Clin Microbiol       Date:  1999-03       Impact factor: 5.948

9.  Improved detection of Candida albicans by PCR in blood of neutropenic mice with systemic candidiasis.

Authors:  A J van Deventer; W H Goessens; A van Belkum; H J van Vliet; E W van Etten; H A Verbrugh
Journal:  J Clin Microbiol       Date:  1995-03       Impact factor: 5.948

10.  Rapid identification of Candida albicans and other human pathogenic yeasts by using short oligonucleotides in a PCR.

Authors:  B M Mannarelli; C P Kurtzman
Journal:  J Clin Microbiol       Date:  1998-06       Impact factor: 5.948
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