Literature DB >> 8118399

Quantitative aspects of the mutant analysis by PCR and restriction enzyme cleavage (MAPREC).

Z Lu1, M P Douthitt, R E Taffs, Y Ran, L P Norwood, K M Chumakov.   

Abstract

Quantitation of virus revertants by PCR and restriction enzyme cleavage may give nonlinear results and, in some cases, produce artifacts caused by nucleotide misincorporation and heteroduplex formation, occurring during PCR. Modifications of the procedure allowed us to overcome these problems and develop a highly sensitive and reliable method of mutant quantitation. This procedure can be used to assess the quality of live vaccines and to study heterogeneity of viral and bacterial populations.

Mesh：

Substances：

Year:  1993        PMID： 8118399     DOI： 10.1101/gr.3.3.176

Source DB:  PubMed          Journal:  PCR Methods Appl        ISSN： 1054-9803


  3 in total

1.  Quantitative mutant analysis of viral quasispecies by chip-based matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry.

Authors:  G Amexis; P Oeth; K Abel; A Ivshina; F Pelloquin; C R Cantor; A Braun; K Chumakov; A Brau
Journal:  Proc Natl Acad Sci U S A       Date:  2001-10-02       Impact factor: 11.205

2.  Quantification of mRNA expression by competitive PCR using non-homologous competitors containing a shifted restriction site.

Authors:  F Watzinger; E Hörth; T Lion
Journal:  Nucleic Acids Res       Date:  2001-06-01       Impact factor: 16.971

3.  PCR engineering of viral quasispecies: a new method to preserve and manipulate genetic diversity of RNA virus populations.

Authors:  K M Chumakov
Journal:  J Virol       Date:  1996-10       Impact factor: 5.103
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.