Literature DB >> 8106596

Development of an isotope-selective high-performance liquid chromatography detector using chemical-reaction-interface mass spectrometry: application to deuterated cortisol metabolites in urine.

Y Teffera1, F P Abramson, M McLean, M Vestal.   

Abstract

An isotope-selective detector for HPLC based on the particle-beam-interface and the chemical-reaction-interface mass spectrometer (CRIMS) principle is described. This paper focuses on the selective detection of deuterium-labeled analytes. The CRIMS product HD is detected as has been previously described for GC-CRIMS. The analytical performance was not affected by analyte structure or solvent composition. Deuterium detection was linear from 20 ng to 490 ng using 2H3-labeled cortisol. Based on this method, the fractional abundance of three labeled metabolites of cortisol were determined in the urine of a patient infused with tracer amounts of deuterated cortisol.

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Year:  1993        PMID： 8106596     DOI： 10.1016/0378-4347(93)80055-9

Source DB:  PubMed          Journal:  J Chromatogr


  3 in total

1.  An inductively coupled plasma-time-of-flight mass spectrometer for elemental analysis. Part III: Analytical performance.

Authors:  D P Myers; G Li; P P Mahoney; G M Hieftje
Journal:  J Am Soc Mass Spectrom       Date:  1995-05       Impact factor: 3.109

2.  Implementation of the chemical reaction interface mass spectrometry technique on a Hewlett-Packard mass-selective detector.

Authors:  H Song; J Kusmierz; F Abramson; M McLean
Journal:  J Am Soc Mass Spectrom       Date:  1994-08       Impact factor: 3.109

3.  Funaria hygrometrica Hedw. elevated tolerance to D2O: its use for the production of highly deuterated metabolites.

Authors:  Fredd Vergara; Misao Itouga; Roberto Gamboa Becerra; Masami Hirai; José Juan Ordaz-Ortiz; Robert Winkler
Journal:  Planta       Date:  2017-10-13       Impact factor: 4.116
  3 in total

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