Fredd Vergara1, Misao Itouga2, Roberto Gamboa Becerra3, Masami Hirai4, José Juan Ordaz-Ortiz3, Robert Winkler5. 1. Metabolic Systems Research Team, RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan. fredd@unam.mx. 2. Plant Productivity Systems Research Group, RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Japan. 3. National Laboratory of Genomics for Biodiversity, CINVESTAV Unidad Irapuato, Irapuato, Mexico. 4. Metabolic Systems Research Team, RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan. 5. Department of Biotechnology and Biochemistry, CINVESTAV Unidad Irapuato, Irapuato, Mexico.
Abstract
MAIN CONCLUSION: The method introduced here to grow F. hygrometrica in high concentrations of D 2 O is an excellent alternative to produce highly deuterated metabolites with broad applications in metabolic studies. Our mass spectrometry experiments strongly indicate the successful incorporation of deuterium into organic compounds. Deuterated metabolites are useful tracers for metabolic studies, yet their wide utilization in research is limited by the multi-step total synthesis required to produce them in the laboratory. Alternatively, deuterated metabolites can be obtained from organisms grown in D2O or deuterated nutrients. This approach also has limitations as D2O in high concentrations negatively affects the survival of most organisms. Here we report the moss Funaria hygrometrica as an unusual high tolerant to D2O in liquid culture. We found that this moss is able to grow in up to 90% D2O, a condition lethal for many eukaryotes. Mass spectrometric analyses of F. hygrometrica extracts showed a strong deuteration pattern. The ability to tolerate high concentrations of D2O together with the development of a rich molecular toolbox makes F. hygrometrica an ideal system for the production of valuable deuterated metabolites.
MAIN CONCLUSION: The method introduced here to grow F. hygrometrica in high concentrations of D 2 O is an excellent alternative to produce highly deuterated metabolites with broad applications in metabolic studies. Our mass spectrometry experiments strongly indicate the successful incorporation of deuterium into organic compounds. Deuterated metabolites are useful tracers for metabolic studies, yet their wide utilization in research is limited by the multi-step total synthesis required to produce them in the laboratory. Alternatively, deuterated metabolites can be obtained from organisms grown in D2O or deuterated nutrients. This approach also has limitations as D2O in high concentrations negatively affects the survival of most organisms. Here we report the moss Funaria hygrometrica as an unusual high tolerant to D2O in liquid culture. We found that this moss is able to grow in up to 90% D2O, a condition lethal for many eukaryotes. Mass spectrometric analyses of F. hygrometrica extracts showed a strong deuteration pattern. The ability to tolerate high concentrations of D2O together with the development of a rich molecular toolbox makes F. hygrometrica an ideal system for the production of valuable deuterated metabolites.
Entities:
Keywords:
Bryophyte; Deuterium; Heavy water; Isotopic labeling; Isotopomer; Mass spectrometry; Moss
Authors: Xiao-Yuan Yang; Wen-Ping Chen; Aaron K Rendahl; Adrian D Hegeman; William M Gray; Jerry D Cohen Journal: Plant J Date: 2010-08 Impact factor: 6.417