Literature DB >> 8105645

A rapid DNA extraction method from culture and clinical samples. Suitable for the detection of human cytomegalovirus by the polymerase chain reaction.

C Zandotti1, X De Lamballerie, C Guignole-Vignoli, C Bollet, P De Micco.   

Abstract

We propose an one-step DNA extraction method suitable for the polymerase chain reaction. This procedure utilizes Chelex 100, a chelating in exchange resin. This technique was compared with a traditional technique (proteinase K lysis, phenol-chloroform extraction and ethanol precipitation) for isolation of human cytomegalovirus DNA from clinical samples. The procedure using Chelex 100 appeared to be a simple and fast extraction method for human cytomegalovirus DNA.

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Year:  1993        PMID： 8105645

Source DB:  PubMed          Journal:  Acta Virol        ISSN： 0001-723X            Impact factor:   1.162


  3 in total

1.  Detection of Herpes simplex virus DNA by real-time PCR.

Authors:  H H Kessler; G Mühlbauer; B Rinner; E Stelzl; A Berger; H W Dörr; B Santner; E Marth; H Rabenau
Journal:  J Clin Microbiol       Date:  2000-07       Impact factor: 5.948

2.  Rapid detection of Mycoplasma pneumoniae by an assay based on PCR and probe hybridization in a nonradioactive microwell plate format.

Authors:  H H Kessler; D E Dodge; K Pierer; K K Young; Y Liao; B I Santner; E Eber; M G Roeger; D Stuenzner; B Sixl-Voigt; E Marth
Journal:  J Clin Microbiol       Date:  1997-06       Impact factor: 5.948

3.  Detection of human bocavirus in hospitalised children.

Authors:  Julia Dina; Astrid Vabret; Stephanie Gouarin; Joelle Petitjean; Julie Lecoq; Jacques Brouard; Alina Arion; Françoise Lafay-Delaire; François Freymuth
Journal:  J Paediatr Child Health       Date:  2009-02-02       Impact factor: 1.954
  3 in total

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