Literature DB >> 8097555

Construction of a basic genetic map for alfalfa using RFLP, RAPD, isozyme and morphological markers.

G B Kiss1, G Csanádi, K Kálmán, P Kaló, L Okrész.   

Abstract

The genetic map for alfalfa presented here has eight linkage groups representing the haploid chromosome set of the Medicago species. The genetic map was constructed by ordering the linkage values of 89 RFLP, RAPD, isozyme and morphological markers collected from a segregating population of 138 individuals. The segregating population is self-mated progeny of an F1 hybrid plant deriving from a cross between the diploid (2n = 2x = 16) yellow-flowered. Medicago sativa ssp. quasifalcata and the diploid (2n = 2x = 16) blue-flowered M. sativa ssp. coerulea. The inheritance of many traits displayed distorted segregation, indicating the presence of lethal loci in the heterozygotic parent plants. In spite of the lack of uniform segregation, linkage groups could be assigned and the order of the markers spanning > 659 centimorgans could be unambiguously determined. This value and the calculated haploid genome size for Medicago (1n = 1x = 1.0 x 10(9) bp) gives a ratio of < 1500 kb per centimorgan.

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Year:  1993        PMID: 8097555     DOI: 10.1007/bf00279539

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN: 0026-8925


  36 in total

1.  Genetic segregation of random amplified polymorphic DNA in diploid cultivated alfalfa.

Authors:  C S Echt; L A Erdahl; T J McCoy
Journal:  Genome       Date:  1992-02       Impact factor: 2.166

2.  Construction of genetic linkage maps in maize and tomato using restriction fragment length polymorphisms.

Authors:  T Helentjaris; M Slocum; S Wright; A Schaefer; J Nienhuis
Journal:  Theor Appl Genet       Date:  1986-09       Impact factor: 5.699

3.  Estimation of recombination frequencies and construction of RFLP linkage maps in plants from crosses between heterozygous parents.

Authors:  E Ritter; C Gebhardt; F Salamini
Journal:  Genetics       Date:  1990-07       Impact factor: 4.562

4.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

5.  LINKAGE-1: a PASCAL computer program for the detection and analysis of genetic linkage.

Authors:  K A Suiter; J F Wendel; J S Case
Journal:  J Hered       Date:  1983 May-Jun       Impact factor: 2.645

6.  Molecular mapping of rice chromosomes.

Authors:  S R McCouch; G Kochert; Z H Yu; Z Y Wang; G S Khush; W R Coffman; S D Tanksley
Journal:  Theor Appl Genet       Date:  1988-12       Impact factor: 5.699

7.  Nodules elicited by Rhizobium meliloti heme mutants are arrested at an early stage of development.

Authors:  R Dickstein; D C Scheirer; W H Fowle; F M Ausubel
Journal:  Mol Gen Genet       Date:  1991-12

8.  The isolation and characterisation of a cDNA clone encoding L-asparaginase from developing seeds of lupin (Lupinus arboreus).

Authors:  T J Lough; B D Reddington; M R Grant; D F Hill; P H Reynolds; K J Farnden
Journal:  Plant Mol Biol       Date:  1992-06       Impact factor: 4.076

Review 9.  Construction of a genetic linkage map in man using restriction fragment length polymorphisms.

Authors:  D Botstein; R L White; M Skolnick; R W Davis
Journal:  Am J Hum Genet       Date:  1980-05       Impact factor: 11.025

10.  The nucleotide sequence of a nodule-specific gene, Nms-25 of Medicago sativa: its primary evolution via exon-shuffling and retrotransposon-mediated DNA rearrangements.

Authors:  Z Végh; E Vincze; R Kadirov; G Tóth; G B Kiss
Journal:  Plant Mol Biol       Date:  1990-08       Impact factor: 4.076

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  27 in total

1.  Excess heterozygosity contributes to genetic map expansion in pea recombinant inbred populations.

Authors:  M R Knox; T H N Ellis
Journal:  Genetics       Date:  2002-10       Impact factor: 4.562

2.  A genetic linkage map of microsatellite, gene-specific and morphological markers in diploid Fragaria.

Authors:  D J Sargent; T M Davis; K R Tobutt; M J Wilkinson; N H Battey; D W Simpson
Journal:  Theor Appl Genet       Date:  2004-07-29       Impact factor: 5.699

Review 3.  Advances in molecular marker techniques and their applications in plant sciences.

Authors:  Milee Agarwal; Neeta Shrivastava; Harish Padh
Journal:  Plant Cell Rep       Date:  2008-02-02       Impact factor: 4.570

4.  A linkage map for sugi (Cryptomeria japonica) based on RFLP, RAPD, and isozyme loci.

Authors:  Y Mukai; Y Suyama; Y Tsumura; T Kawahara; H Yoshimaru; T Kondo; N Tomaru; N Kuramoto; M Murai
Journal:  Theor Appl Genet       Date:  1995-05       Impact factor: 5.699

5.  Dwarf plants of diploid Medicago sativa carry a mutation in the gibberellin 3-beta-hydroxylase gene.

Authors:  Agnes Dalmadi; Péter Kaló; Júlia Jakab; Anikó Saskoi; Tünde Petrovics; Gábor Deák; György Botond Kiss
Journal:  Plant Cell Rep       Date:  2008-05-27       Impact factor: 4.570

6.  Relationships among genetic distance, forage yield and heterozygosity in isogenic diploid and tetraploid alfalfa populations.

Authors:  K K Kidwell; E T Bingham; D R Woodfield; T C Osborn
Journal:  Theor Appl Genet       Date:  1994-10       Impact factor: 5.699

7.  Prevalence of segregation distortion in diploid alfalfa and its implications for genetics and breeding applications.

Authors:  Xuehui Li; Xiaojuan Wang; Yanling Wei; E Charles Brummer
Journal:  Theor Appl Genet       Date:  2011-05-31       Impact factor: 5.699

8.  A sequence-based genetic map of Medicago truncatula and comparison of marker colinearity with M. sativa.

Authors:  Hong-Kyu Choi; Dongjin Kim; Taesik Uhm; Eric Limpens; Hyunju Lim; Jeong-Hwan Mun; Peter Kalo; R Varma Penmetsa; Andrea Seres; Olga Kulikova; Bruce A Roe; Ton Bisseling; Gyorgy B Kiss; Douglas R Cook
Journal:  Genetics       Date:  2004-03       Impact factor: 4.562

9.  An expressed sequence tag SSR map of tetraploid alfalfa (Medicago sativa L.).

Authors:  M K Sledge; I M Ray; G Jiang
Journal:  Theor Appl Genet       Date:  2005-08-02       Impact factor: 5.699

10.  Strategies to obtain stable transgenic plants from non-embryogenic lines: complementation of the nn1 mutation of the NORK gene in Medicago sativa MN1008.

Authors:  Ariana Perhald; Gabriella Endre; Zoltan Kevei; Gyorgy B Kiss; Attila Kereszt
Journal:  Plant Cell Rep       Date:  2006-03-14       Impact factor: 4.570

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