Literature DB >> 1572530

Genetic segregation of random amplified polymorphic DNA in diploid cultivated alfalfa.

C S Echt1, L A Erdahl, T J McCoy.   

Abstract

Polymerase chain reaction was used, with single 10-mer primers of arbitrary sequence, to amplify random regions of genomic DNA from a diploid cultivated alfalfa backcross population. Segregation of the random amplified polymorphic DNA (RAPD) fragments was analysed to determine if RAPD markers are suitable for use as genetic markers. Of the 19 primers tested, 13 amplified a total of 37 polymorphic fragments, of which 28 (76%) segregated as dominant Mendelian traits. RAPD markers appear useful for the rapid development of genetic information in species like alfalfa where little information currently exists or is difficult to obtain.

Entities:  

Mesh：

Substances：

Year:  1992        PMID： 1572530     DOI： 10.1139/g92-014

Source DB:  PubMed          Journal:  Genome        ISSN： 0831-2796            Impact factor:   2.166


  22 in total

1.  Randomly amplified polymorphic DNA markers are superior to somatic incompatibility tests for discriminating genotypes in natural populations of the ectomycorrhizal fungus Suillus granulatus.

Authors:  K M Jacobson; O K Miller; B J Turner
Journal:  Proc Natl Acad Sci U S A       Date:  1993-10-01       Impact factor: 11.205

2.  Genetic variation of Oplopanax elatus (Araliaceae) populations estimated using DNA molecular markers.

Authors:  G D Reunova; I L Kats; Yu N Zhuravlev
Journal:  Dokl Biol Sci       Date:  2010-08-17

3.  Competition as a source of errors in RAPD analysis.

Authors:  C Halldén; M Hansen; N O Nilsson; A Hjerdin; T Säll
Journal:  Theor Appl Genet       Date:  1996-12       Impact factor: 5.699

4.  Genetic validity of RAPD markers at the intra- and inter-specific level in wild Brassica species with n=9.

Authors:  C Lannér; T Bryngelsson; M Gustafsson
Journal:  Theor Appl Genet       Date:  1996-07       Impact factor: 5.699

5.  Random amplified polymorphic DNA (RAPD) markers in hop, Humulus lupulus: level of genetic variability and segregation in F1 progeny.

Authors:  M Pillay; S T Kenny
Journal:  Theor Appl Genet       Date:  1996-03       Impact factor: 5.699

6.  Identification of RAPD markers linked to a major rust resistance gene block in common bean.

Authors:  S D Haley; P N Miklas; J R Stavely; J Byrum; J D Kelly
Journal:  Theor Appl Genet       Date:  1993-05       Impact factor: 5.699

7.  Random amplified polymorphic DNA and pedigree relationships in spring barley.

Authors:  N A Tinker; M G Fortin; D E Mather
Journal:  Theor Appl Genet       Date:  1993-02       Impact factor: 5.699

8.  Inheritance of RAPDs in F1 hybrids of corn.

Authors:  M Heun; T Helentjaris
Journal:  Theor Appl Genet       Date:  1993-02       Impact factor: 5.699

9.  Origin, distribution and mapping of RAPD markers from wildPetunia species inPetunia hybrida Hort lines.

Authors:  D Peltier; E Farcy; H Dulieu; A Bervillé
Journal:  Theor Appl Genet       Date:  1994-08       Impact factor: 5.699

10.  Heteroduplex molecules formed between allelic sequences cause nonparental RAPD bands.

Authors:  M A Ayliffe; G J Lawrence; J G Ellis; A J Pryor
Journal:  Nucleic Acids Res       Date:  1994-05-11       Impact factor: 16.971
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.