Literature DB >> 8074530

Nucleotide sequence and expression of a novel pectate lyase gene (pel-3) and a closely linked endopolygalacturonase gene (peh-1) of Erwinia carotovora subsp. carotovora 71.

Y Liu1, A Chatterjee, A K Chatterjee.   

Abstract

Our previous genetic analysis (J. W. Willis, J. K. Engwall, and A. K. Chatterjee, Phytopathology 77:1199-1205, 1987) had revealed a tight linkage between pel-3 (pel, pectate lyase gene) and peh-1 (peh, polygalacturonase gene) within the chromosome of Erwinia carotovora subsp. carotovora 71. Nucleotide sequencing, transcript assays, and expression of enzymatic activities in Escherichia coli have now confirmed that a 3,500-bp segment contains the open reading frames (ORFs) for Pel-3 and Peh-1. The 1,041-bp pel-3 ORF and the 1,206-bp peh-1 ORF are separated by a 579-bp sequence. The genes are transcribed divergently from their own promoters. In E. coli and E. carotovora subsp. carotovora 71, peh-1 is better expressed than pel-3. However, plant signals activate the expression of both the genes in E. carotovora subsp. carotovora. A consensus integration host factor (IHF)-binding sequence upstream of pel-3 appears physiologically significant, since pel-3 promoter activity is higher in an E. coli IHF+ strain than in an IHF- strain. While peh-1 has extensive homology with plant and bacterial peh genes, pel-3 appears not to have significant homology with the pel genes belonging to the pelBC, pelADE, or periplasmic pel families. Pel-3 also is unusual in that it is predicted to contain an ATP- and GTP-binding site motif A (P-loop) not found in the other Pels.

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Year:  1994        PMID: 8074530      PMCID: PMC201682          DOI: 10.1128/aem.60.7.2545-2552.1994

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  39 in total

1.  Regulation of a stylar transmitting tissue-specific gene in wild-type and transgenic tomato and tobacco.

Authors:  K A Budelier; A G Smith; C S Gasser
Journal:  Mol Gen Genet       Date:  1990-11

2.  Activity stain for rapid characterization of pectic enzymes in isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gels.

Authors:  J L Ried; A Collmer
Journal:  Appl Environ Microbiol       Date:  1985-09       Impact factor: 4.792

3.  Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria.

Authors:  V de Lorenzo; M Herrero; U Jakubzik; K N Timmis
Journal:  J Bacteriol       Date:  1990-11       Impact factor: 3.490

4.  Improved plasmid vectors for the isolation of translational lac gene fusions.

Authors:  N P Minton
Journal:  Gene       Date:  1984-11       Impact factor: 3.688

5.  Cloning and expression in Escherichia coli of pectinase genes of Erwinia carotovora subsp. carotovora.

Authors:  R T Zink; A K Chatterjee
Journal:  Appl Environ Microbiol       Date:  1985-03       Impact factor: 4.792

6.  Relationship between the pel genes of the pelADE cluster in Erwinia chrysanthemi strain B374.

Authors:  F van Gijsegem
Journal:  Mol Microbiol       Date:  1989-10       Impact factor: 3.501

7.  Nucleotide sequence and molecular characterization of pnlA, the structural gene for damage-inducible pectin lyase of Erwinia carotovora subsp. carotovora 71.

Authors:  A Chatterjee; J L McEvoy; J P Chambost; F Blasco; A K Chatterjee
Journal:  J Bacteriol       Date:  1991-03       Impact factor: 3.490

8.  recA is required in the induction of pectin lyase and carotovoricin in Erwinia carotovora subsp. carotovora.

Authors:  R T Zink; J K Engwall; J L McEvoy; A K Chatterjee
Journal:  J Bacteriol       Date:  1985-10       Impact factor: 3.490

9.  Analysis of the regulation of the pelBC genes in Erwinia chrysanthemi 3937.

Authors:  N Hugouvieux-Cotte-Pattat; J Robert-Baudouy
Journal:  Mol Microbiol       Date:  1992-08       Impact factor: 3.501

10.  Nucleotide sequences of the Erwinia chrysanthemi ogl and pelE genes negatively regulated by the kdgR gene product.

Authors:  S Reverchon; Y Huang; C Bourson; J Robert-Baudouy
Journal:  Gene       Date:  1989-12-21       Impact factor: 3.688

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  23 in total

1.  Comparative analysis of two classes of quorum-sensing signaling systems that control production of extracellular proteins and secondary metabolites in Erwinia carotovora subspecies.

Authors:  Asita Chatterjee; Yaya Cui; Hiroaki Hasegawa; Nathan Leigh; Vaishali Dixit; Arun K Chatterjee
Journal:  J Bacteriol       Date:  2005-12       Impact factor: 3.490

2.  ExpR, a LuxR homolog of Erwinia carotovora subsp. carotovora, activates transcription of rsmA, which specifies a global regulatory RNA-binding protein.

Authors:  Yaya Cui; Asita Chatterjee; Hiroaki Hasegawa; Vaishali Dixit; Nathan Leigh; Arun K Chatterjee
Journal:  J Bacteriol       Date:  2005-07       Impact factor: 3.490

3.  Elevated temperature enhances virulence of Erwinia carotovora subsp. carotovora strain EC153 to plants and stimulates production of the quorum sensing signal, N-acyl homoserine lactone, and extracellular proteins.

Authors:  H Hasegawa; A Chatterjee; Y Cui; A K Chatterjee
Journal:  Appl Environ Microbiol       Date:  2005-08       Impact factor: 4.792

4.  Use of Tn5tac1 to clone a pel gene encoding a highly alkaline, asparagine-rich pectate lyase isozyme from an Erwinia chrysanthemi EC16 mutant with deletions affecting the major pectate lyase isozymes.

Authors:  J R Alfano; J H Ham; A Collmer
Journal:  J Bacteriol       Date:  1995-08       Impact factor: 3.490

5.  rsmC of the soft-rotting bacterium Erwinia carotovora subsp. carotovora negatively controls extracellular enzyme and harpin(Ecc) production and virulence by modulating levels of regulatory RNA (rsmB) and RNA-binding protein (RsmA).

Authors:  Y Cui; A Mukherjee; C K Dumenyo; Y Liu; A K Chatterjee
Journal:  J Bacteriol       Date:  1999-10       Impact factor: 3.490

6.  Erwinia carotovora subspecies produce duplicate variants of ExpR, LuxR homologs that activate rsmA transcription but differ in their interactions with N-acylhomoserine lactone signals.

Authors:  Yaya Cui; Asita Chatterjee; Hiroaki Hasegawa; Arun K Chatterjee
Journal:  J Bacteriol       Date:  2006-07       Impact factor: 3.490

7.  Inactivation of rsmA leads to overproduction of extracellular pectinases, cellulases, and proteases in Erwinia carotovora subsp. carotovora in the absence of the starvation/cell density-sensing signal, N-(3-oxohexanoyl)-L-homoserine lactone.

Authors:  A Chatterjee; Y Cui; Y Liu; C K Dumenyo; A K Chatterjee
Journal:  Appl Environ Microbiol       Date:  1995-05       Impact factor: 4.792

8.  RsmC of Erwinia carotovora subsp. carotovora negatively controls motility, extracellular protein production, and virulence by binding FlhD and modulating transcriptional activity of the master regulator, FlhDC.

Authors:  Asita Chatterjee; Yaya Cui; Arun K Chatterjee
Journal:  J Bacteriol       Date:  2009-05-15       Impact factor: 3.490

9.  RsmA and the quorum-sensing signal, N-[3-oxohexanoyl]-L-homoserine lactone, control the levels of rsmB RNA in Erwinia carotovora subsp. carotovora by affecting its stability.

Authors:  Asita Chatterjee; Yaya Cui; Arun K Chatterjee
Journal:  J Bacteriol       Date:  2002-08       Impact factor: 3.490

10.  Regulatory network controlling extracellular proteins in Erwinia carotovora subsp. carotovora: FlhDC, the master regulator of flagellar genes, activates rsmB regulatory RNA production by affecting gacA and hexA (lrhA) expression.

Authors:  Yaya Cui; Asita Chatterjee; Hailian Yang; Arun K Chatterjee
Journal:  J Bacteriol       Date:  2008-04-25       Impact factor: 3.490

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