Progressive hair cell loss induced by toluene exposure.

Rats were exposed to toluene by inhalation (1400 ppm, 16 h/d, 8 days) and sacrificed for morphological investigations at 3 and 5 days after the start of the exposure, and 4 days and 6 weeks after the end of the exposure. The cochleae were removed and prepared for light microscopy and scanning electron microscopy. After 3 days of toluene exposure no loss of hair cells was found. A slight loss in the third row outer hair cells was observed after 5 days of exposure. Four days after the 8-day long exposure a loss of hair cells was found in all 3 rows of outer hair cells, mainly in the middle and upper turns of the cochlea. Six weeks post-exposure the damage on the hair cells had progressed towards the basal part of the cochlea, and a 50-100% loss of outer hair cells together with some loss of inner hair cells were seen. A fairly good correlation was found between the frequency regions showing loss of hair cells and the threshold shifts previously measured by auditory brainstem responses and distortion product otoacoustic emissions in the same rats at corresponding times (Johnson and Canlon, 1994). These results indicate that the outer hair cells in the middle frequency region of the cochlea, were primarily affected by toluene exposure. However, after a long post-exposure period the damage extended basally and apically and some damage to the inner hair cells was seen.