Literature DB >> 8068937

Hypersensitive site 5 of the human beta locus control region functions as a chromatin insulator.

Q Li1, G Stamatoyannopoulos.   

Abstract

The human beta locus control region (LCR) consists of five DNAse I hypersensitive sites (HS), four of which are erythroid specific and one, the further upstream located 5'HS5, is constitutive. To characterize the function of 5'HS5 we analyzed globin gene expression of various constructs containing HS3 as an enhancer, HS5, and the beta gene as a reporter. Expression was analyzed in stably transfected MEL cells. We found that the enhancing effect of hypersensitive site 3 is blocked when the HS5 is interposed between HS3 and the beta globin gene. These data suggest that the human 5'HS5 has the properties of a chromatin insulator.

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Year:  1994        PMID： 8068937

Source DB:  PubMed          Journal:  Blood        ISSN： 0006-4971            Impact factor:   22.113


  30 in total

1.  The polyoma virus enhancer cannot substitute for DNase I core hypersensitive sites 2-4 in the human beta-globin LCR.

Authors:  K Tanimoto; Q Liu; J Bungert; J D Engel
Journal:  Nucleic Acids Res       Date:  1999-08-01       Impact factor: 16.971

2.  Structural and functional conservation at the boundaries of the chicken beta-globin domain.

Authors:  N Saitoh; A C Bell; F Recillas-Targa; A G West; M Simpson; M Pikaart; G Felsenfeld
Journal:  EMBO J       Date:  2000-05-15       Impact factor: 11.598

Review 3.  Locus control regions.

Authors:  Qiliang Li; Kenneth R Peterson; Xiangdong Fang; George Stamatoyannopoulos
Journal:  Blood       Date:  2002-11-01       Impact factor: 22.113

4.  A complex chromatin landscape revealed by patterns of nuclease sensitivity and histone modification within the mouse beta-globin locus.

Authors:  Michael Bulger; Dirk Schübeler; M A Bender; Joan Hamilton; Catherine M Farrell; Ross C Hardison; Mark Groudine
Journal:  Mol Cell Biol       Date:  2003-08       Impact factor: 4.272

5.  The ERV-9 LTR enhancer is not blocked by the HS5 insulator and synthesizes through the HS5 site non-coding, long RNAs that regulate LTR enhancer function.

Authors:  Jianhua Ling; Wenhu Pi; Xiuping Yu; Chikh Bengra; Qiaoming Long; Huaqian Jin; Andreas Seyfang; Dorothy Tuan
Journal:  Nucleic Acids Res       Date:  2003-08-01       Impact factor: 16.971

6.  Genome-wide in silico mapping of scaffold/matrix attachment regions in Arabidopsis suggests correlation of intragenic scaffold/matrix attachment regions with gene expression.

Authors:  Stephen Rudd; Matthias Frisch; Korbinian Grote; Blake C Meyers; Klaus Mayer; Thomas Werner
Journal:  Plant Physiol       Date:  2004-06       Impact factor: 8.340

7.  Sequences within and flanking hypersensitive sites 3 and 2 of the beta-globin locus control region required for synergistic versus additive interaction with the epsilon-globin gene promoter.

Authors:  J D Jackson; W Miller; R C Hardison
Journal:  Nucleic Acids Res       Date:  1996-11-01       Impact factor: 16.971

8.  Heterogeneous nuclear ribonucleoprotein C1/C2, MeCP1, and SWI/SNF form a chromatin remodeling complex at the beta-globin locus control region.

Authors:  Milind C Mahajan; Geeta J Narlikar; Gokul Boyapaty; Robert E Kingston; Sherman M Weissman
Journal:  Proc Natl Acad Sci U S A       Date:  2005-10-10       Impact factor: 11.205

9.  Quantification of DNaseI-sensitivity by real-time PCR: quantitative analysis of DNaseI-hypersensitivity of the mouse beta-globin LCR.

Authors:  M McArthur; S Gerum; G Stamatoyannopoulos
Journal:  J Mol Biol       Date:  2001-10-12       Impact factor: 5.469

10.  NF-E2 disrupts chromatin structure at human beta-globin locus control region hypersensitive site 2 in vitro.

Authors:  J A Armstrong; B M Emerson
Journal:  Mol Cell Biol       Date:  1996-10       Impact factor: 4.272
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