Literature DB >> 8063428

Shuttle mutagenesis of Legionella pneumophila: identification of a gene associated with host cell cytopathicity.

J Arroyo1, M C Hurley, M Wolf, M S McClain, B I Eisenstein, N C Engleberg.   

Abstract

We performed shuttle mutagenesis of Legionella pneumophila. Mutants were screened for reduced cellular infectivity. Approximately 10% of the mutants had decreased cytopathicity. The DNA sequence of one locus was determined; the inferred amino acid sequence revealed homology with transport proteins including Escherichia coli TolC, Bordetella pertussis CyaE, and Alcaligenes eutrophus CzcC and CnrC.

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Year:  1994        PMID： 8063428      PMCID： PMC303072          DOI： 10.1128/iai.62.9.4075-4080.1994

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  25 in total

1.  A complementation analysis of the restriction and modification of DNA in Escherichia coli.

Authors:  H W Boyer; D Roulland-Dussoix
Journal:  J Mol Biol       Date:  1969-05-14       Impact factor: 5.469

2.  The construction of a cloning vector designed for gene replacement in Bordetella pertussis.

Authors:  S Stibitz; W Black; S Falkow
Journal:  Gene       Date:  1986       Impact factor: 3.688

3.  Primary structure of the tolC gene that codes for an outer membrane protein of Escherichia coli K12.

Authors:  J Hackett; P Reeves
Journal:  Nucleic Acids Res       Date:  1983-09-24       Impact factor: 16.971

4.  Shuttle mutagenesis: a method of transposon mutagenesis for Saccharomyces cerevisiae.

Authors:  H S Seifert; E Y Chen; M So; F Heffron
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205

5.  Conjugal transfer of bacterial chromosomes mediated by the RK2 plasmid transfer origin cloned into transposon Tn5.

Authors:  E A Yakobson; D G Guiney
Journal:  J Bacteriol       Date:  1984-10       Impact factor: 3.490

6.  Purification and characterization of an extracellular protease of Legionella pneumophila.

Authors:  L A Dreyfus; B H Iglewski
Journal:  Infect Immun       Date:  1986-03       Impact factor: 3.441

7.  Characterization of the inducible nickel and cobalt resistance determinant cnr from pMOL28 of Alcaligenes eutrophus CH34.

Authors:  H Liesegang; K Lemke; R A Siddiqui; H G Schlegel
Journal:  J Bacteriol       Date:  1993-02       Impact factor: 3.490

8.  Metal requirements of Legionella pneumophila.

Authors:  M W Reeves; L Pine; S H Hutner; J R George; W K Harrell
Journal:  J Clin Microbiol       Date:  1981-04       Impact factor: 5.948

9.  Studies on transformation of Escherichia coli with plasmids.

Authors:  D Hanahan
Journal:  J Mol Biol       Date:  1983-06-05       Impact factor: 5.469

10.  TnphoA: a transposon probe for protein export signals.

Authors:  C Manoil; J Beckwith
Journal:  Proc Natl Acad Sci U S A       Date:  1985-12       Impact factor: 11.205
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  13 in total

1.  Legionella pneumophila entry gene rtxA is involved in virulence.

Authors:  S L Cirillo; L E Bermudez; S H El-Etr; G E Duhamel; J D Cirillo
Journal:  Infect Immun       Date:  2001-01       Impact factor: 3.441

2.  Natural competence for DNA transformation by Legionella pneumophila and its association with expression of type IV pili.

Authors:  B J Stone; Y A Kwaik
Journal:  J Bacteriol       Date:  1999-03       Impact factor: 3.490

3.  Identification of Legionella pneumophila mutants that have aberrant intracellular fates.

Authors:  M S Swanson; R R Isberg
Journal:  Infect Immun       Date:  1996-07       Impact factor: 3.441

4.  Identification of Legionella pneumophila genes important for infection of amoebas by signature-tagged mutagenesis.

Authors:  A H Polesky; J T Ross; S Falkow; L S Tompkins
Journal:  Infect Immun       Date:  2001-02       Impact factor: 3.441

5.  Identification of the aspartate-beta-semialdehyde dehydrogenase gene of Legionella pneumophila and characterization of a null mutant.

Authors:  O S Harb; Y Abu Kwaik
Journal:  Infect Immun       Date:  1998-05       Impact factor: 3.441

6.  Legionella pneumophila mutants that are defective for iron acquisition and assimilation and intracellular infection.

Authors:  C D Pope; W O'Connell; N P Cianciotto
Journal:  Infect Immun       Date:  1996-02       Impact factor: 3.441

7.  Type II protein secretion is a subset of the PilD-dependent processes that facilitate intracellular infection by Legionella pneumophila.

Authors:  O Rossier; N P Cianciotto
Journal:  Infect Immun       Date:  2001-04       Impact factor: 3.441

8.  lvgA, a novel Legionella pneumophila virulence factor.

Authors:  Paul H Edelstein; Baofeng Hu; Futoshi Higa; Martha A C Edelstein
Journal:  Infect Immun       Date:  2003-05       Impact factor: 3.441

9.  Induced expression of the Legionella pneumophila gene encoding a 20-kilodalton protein during intracellular infection.

Authors:  Y Abu Kwaik
Journal:  Infect Immun       Date:  1998-01       Impact factor: 3.441

10.  The czc operon of Alcaligenes eutrophus CH34: from resistance mechanism to the removal of heavy metals.

Authors:  L Diels; Q Dong; D van der Lelie; W Baeyens; M Mergeay
Journal:  J Ind Microbiol       Date:  1995-02
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