Investigations of mannan and glucan in the cell wall of Candida boidinii cultivated on methanol.

The polysaccharide components (mannan and glucan) in the cell wall of Candida boidinii M 363 grown on methanol and glucose as control were investigated using electron microscopy, cytochemical and biochemical methods. An ultrastructural rearrangement of the polymers in the cell wall of yeasts cultivated on methanol in comparison to those cultivated on glucose was established. The morphological changes correlate to the quantitative changes in the polysaccharide constituents of the cell wall. The forming and the role of thiosemihydrocarbazide (TSHC)--negative zones in the Candida boidinii cell wall cultivated on methanol media are discussed.