Literature DB >> 8058826

A Clostridium perfringens vector for the selection of promoters.

C Matsushita1, O Matsushita, M Koyama, A Okabe.   

Abstract

A promoter selection vector for Clostridium perfringens genes was constructed from a C. perfringens-Escherichia coli shuttle vector, pJIR418. The plasmid carries a promoterless chloramphenicol acetyltransferase gene (catP), derived from pIP401, downstream of the multiple cloning sites of pUC18. When a promoter region of the phospholipase C gene was inserted into one of the cloning sites, derivatives of C. perfringens strain 13 carrying the resultant plasmid acquired resistance to chloramphenicol. This plasmid should be useful reporter system for C. perfringens genes.

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Year:  1994        PMID： 8058826     DOI： 10.1006/plas.1994.1035

Source DB:  PubMed          Journal:  Plasmid        ISSN： 0147-619X            Impact factor:   3.466


  10 in total

1.  Transcriptional analysis of the tet(P) operon from Clostridium perfringens.

Authors:  P A Johanesen; D Lyras; T L Bannam; J I Rood
Journal:  J Bacteriol       Date:  2001-12       Impact factor: 3.490

2.  Characterization and development of two reporter gene systems for Clostridium acetobutylicum.

Authors:  Lothar Feustel; Stephan Nakotte; Peter Dürre
Journal:  Appl Environ Microbiol       Date:  2004-02       Impact factor: 4.792

3.  Development and application of a method for counterselectable in-frame deletion in Clostridium perfringens.

Authors:  Hirofumi Nariya; Shigeru Miyata; Motoo Suzuki; Eiji Tamai; Akinobu Okabe
Journal:  Appl Environ Microbiol       Date:  2010-12-23       Impact factor: 4.792

4.  Development and characterization of a xylose-inducible gene expression system for Clostridium perfringens.

Authors:  Hirofumi Nariya; Shigeru Miyata; Tomomi Kuwahara; Akinobu Okabe
Journal:  Appl Environ Microbiol       Date:  2011-09-30       Impact factor: 4.792

5.  Development and characterization of a gene expression reporter system for Clostridium acetobutylicum ATCC 824.

Authors:  S B Tummala; N E Welker; E T Papoutsakis
Journal:  Appl Environ Microbiol       Date:  1999-09       Impact factor: 4.792

6.  Transcriptional regulation of the Clostridium cellulolyticum cip-cel operon: a complex mechanism involving a catabolite-responsive element.

Authors:  Laetitia Abdou; Céline Boileau; Pascale de Philip; Sandrine Pagès; Henri-Pierre Fiérobe; Chantal Tardif
Journal:  J Bacteriol       Date:  2007-12-21       Impact factor: 3.490

7.  Optimized clostridium-directed enzyme prodrug therapy improves the antitumor activity of the novel DNA cross-linking agent PR-104.

Authors:  Shie-Chau Liu; G-One Ahn; Mitomu Kioi; Mary-Jo Dorie; Adam V Patterson; J Martin Brown
Journal:  Cancer Res       Date:  2008-10-01       Impact factor: 12.701

8.  Role of alpha-toxin in Clostridium perfringens infection determined by using recombinants of C. perfringens and Bacillus subtilis.

Authors:  M Ninomiya; O Matsushita; J Minami; H Sakamoto; M Nakano; A Okabe
Journal:  Infect Immun       Date:  1994-11       Impact factor: 3.441

9.  Purification, characterization, and primary structure of Clostridium perfringens lambda-toxin, a thermolysin-like metalloprotease.

Authors:  F Jin; O Matsushita; S Katayama; S Jin; C Matsushita; J Minami; A Okabe
Journal:  Infect Immun       Date:  1996-01       Impact factor: 3.441

10.  Development of a sensitive gene expression reporter system and an inducible promoter-repressor system for Clostridium acetobutylicum.

Authors:  Laurence Girbal; Isabelle Mortier-Barrière; Frédéric Raynaud; Céline Rouanet; Christian Croux; Philippe Soucaille
Journal:  Appl Environ Microbiol       Date:  2003-08       Impact factor: 4.792
  10 in total

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