Literature DB >> 21965407

Development and characterization of a xylose-inducible gene expression system for Clostridium perfringens.

Hirofumi Nariya1, Shigeru Miyata, Tomomi Kuwahara, Akinobu Okabe.   

Abstract

A xylose-inducible gene expression vector for Clostridium perfringens was developed. Plasmid pXCH contains a chromosomal region from Clostridium difficile (xylR-P(xy)(lB)): xylR, encoding the xylose repressor, xylO, the xyl operator sequence, and P(xylB), the divergent promoter upstream of xylBA encoding xylulo kinase and xylose isomerase. pXCH allows tightly regulated expression of the chloramphenicol acetyltransferase reporter and the α-toxin genes in response to the inducer concentration. Thus, pXCH could constitute a new valuable genetic tool for study of C. perfringens.

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Year:  2011        PMID： 21965407      PMCID： PMC3233060          DOI： 10.1128/AEM.05668-11

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  14 in total

1.  The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome.

Authors:  Mohammed Sebaihia; Brendan W Wren; Peter Mullany; Neil F Fairweather; Nigel Minton; Richard Stabler; Nicholas R Thomson; Adam P Roberts; Ana M Cerdeño-Tárraga; Hongmei Wang; Matthew T G Holden; Anne Wright; Carol Churcher; Michael A Quail; Stephen Baker; Nathalie Bason; Karen Brooks; Tracey Chillingworth; Ann Cronin; Paul Davis; Linda Dowd; Audrey Fraser; Theresa Feltwell; Zahra Hance; Simon Holroyd; Kay Jagels; Sharon Moule; Karen Mungall; Claire Price; Ester Rabbinowitsch; Sarah Sharp; Mark Simmonds; Kim Stevens; Louise Unwin; Sally Whithead; Bruno Dupuy; Gordon Dougan; Bart Barrell; Julian Parkhill
Journal:  Nat Genet       Date:  2006-06-25       Impact factor: 38.330

2.  Identification and characterization of a putative endolysin encoded by episomal phage phiSM101 of Clostridium perfringens.

Authors:  Hirofumi Nariya; Shigeru Miyata; Eiji Tamai; Hiroshi Sekiya; Jun Maki; Akinobu Okabe
Journal:  Appl Microbiol Biotechnol       Date:  2011-04-12       Impact factor: 4.813

3.  Transcription of the xyl operon is controlled in Bacillus subtilis by tandem overlapping operators spaced by four base-pairs.

Authors:  M K Dahl; J Degenkolb; W Hillen
Journal:  J Mol Biol       Date:  1994-10-28       Impact factor: 5.469

4.  Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater.

Authors:  Tohru Shimizu; Kaori Ohtani; Hideki Hirakawa; Kenshiro Ohshima; Atsushi Yamashita; Tadayoshi Shiba; Naotake Ogasawara; Masahira Hattori; Satoru Kuhara; Hideo Hayashi
Journal:  Proc Natl Acad Sci U S A       Date:  2002-01-15       Impact factor: 11.205

5.  Development and characterization of a xylose-dependent system for expression of cloned genes in Bacillus subtilis: conditional complementation of a teichoic acid mutant.

Authors:  A P Bhavsar; X Zhao; E D Brown
Journal:  Appl Environ Microbiol       Date:  2001-01       Impact factor: 4.792

6.  Absolute and relative QPCR quantification of plasmid copy number in Escherichia coli.

Authors:  Changsoo Lee; Jaai Kim; Seung Gu Shin; Seokhwan Hwang
Journal:  J Biotechnol       Date:  2006-01-18       Impact factor: 3.307

7.  High-level expression of his-tagged clostridial collagenase in Clostridium perfringens.

Authors:  Eiji Tamai; Shigeru Miyata; Hiroaki Tanaka; Hirofumi Nariya; Motoo Suzuki; Osamu Matsushita; Naoya Hatano; Akinobu Okabe
Journal:  Appl Microbiol Biotechnol       Date:  2008-07-16       Impact factor: 4.813

8.  A Clostridium perfringens vector for the selection of promoters.

Authors:  C Matsushita; O Matsushita; M Koyama; A Okabe
Journal:  Plasmid       Date:  1994-05       Impact factor: 3.466

9.  A novel type of DNA curvature present in a Clostridium perfringens ferredoxin gene: characterization and role in gene expression.

Authors:  Masato Kaji; Osamu Matsushita; Eiji Tamai; Shigeru Miyata; Yuki Taniguchi; Seiko Shimamoto; Seiichi Katayama; Shushi Morita; Akinobu Okabe
Journal:  Microbiology (Reading)       Date:  2003-11       Impact factor: 2.777

10.  Construction of a sequenced Clostridium perfringens-Escherichia coli shuttle plasmid.

Authors:  J Sloan; T A Warner; P T Scott; T L Bannam; D I Berryman; J I Rood
Journal:  Plasmid       Date:  1992-05       Impact factor: 3.466
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  12 in total

1.  Utility of the clostridial site-specific recombinase TnpX to clone toxic-product-encoding genes and selectively remove genomic DNA fragments.

Authors:  Vicki Adams; Radhika Bantwal; Lauren Stevenson; Jackie K Cheung; Milena M Awad; Joel Nicholson; Glen P Carter; Kate E Mackin; Julian I Rood; Dena Lyras
Journal:  Appl Environ Microbiol       Date:  2014-06       Impact factor: 4.792

2.  A Xylose-Inducible Expression System and a CRISPR Interference Plasmid for Targeted Knockdown of Gene Expression in Clostridioides difficile.

Authors:  Ute Müh; Anthony G Pannullo; David S Weiss; Craig D Ellermeier
Journal:  J Bacteriol       Date:  2019-06-21       Impact factor: 3.490

3.  Lactose-inducible system for metabolic engineering of Clostridium ljungdahlii.

Authors:  Areen Banerjee; Ching Leang; Toshiyuki Ueki; Kelly P Nevin; Derek R Lovley
Journal:  Appl Environ Microbiol       Date:  2014-02-07       Impact factor: 4.792

4.  Cyclic Di-GMP Binding by an Assembly ATPase (PilB2) and Control of Type IV Pilin Polymerization in the Gram-Positive Pathogen Clostridium perfringens.

Authors:  William A Hendrick; Mona W Orr; Samantha R Murray; Vincent T Lee; Stephen B Melville
Journal:  J Bacteriol       Date:  2017-04-25       Impact factor: 3.490

5.  Examination of Genetic Control Elements in the Phototrophic Firmicute Heliomicrobium modesticaldum.

Authors:  Alexandria M Layton; Kevin E Redding
Journal:  Microorganisms       Date:  2022-04-22

6.  Xylose-Inducible Promoter Tools for Pseudomonas Species and Their Use in Implicating a Role for the Type II Secretion System Protein XcpQ in the Inhibition of Corneal Epithelial Wound Closure.

Authors:  Jake D Callaghan; Nicholas A Stella; Kara M Lehner; Benjamin R Treat; Kimberly M Brothers; Anthony J St Leger; Robert M Q Shanks
Journal:  Appl Environ Microbiol       Date:  2020-07-02       Impact factor: 4.792

7.  The Fatty Acid Synthesis Protein Enoyl-ACP Reductase II (FabK) is a Target for Narrow-Spectrum Antibacterials for Clostridium difficile Infection.

Authors:  Ravi K R Marreddy; Xiaoqian Wu; Madhab Sapkota; Allan M Prior; Jesse A Jones; Dianqing Sun; Kirk E Hevener; Julian G Hurdle
Journal:  ACS Infect Dis       Date:  2018-12-13       Impact factor: 5.084

8.  A novel arabinose-inducible genetic operation system developed for Clostridium cellulolyticum.

Authors:  Jie Zhang; Ya-Jun Liu; Gu-Zhen Cui; Qiu Cui
Journal:  Biotechnol Biofuels       Date:  2015-03-04       Impact factor: 6.040

Review 9.  Recent Developments of the Synthetic Biology Toolkit for Clostridium.

Authors:  Rochelle C Joseph; Nancy M Kim; Nicholas R Sandoval
Journal:  Front Microbiol       Date:  2018-02-12       Impact factor: 5.640

10.  Temperature-regulated heterogeneous extracellular matrix gene expression defines biofilm morphology in Clostridium perfringens.

Authors:  Nozomu Obana; Kouji Nakamura; Nobuhiko Nomura
Journal:  NPJ Biofilms Microbiomes       Date:  2020-07-31       Impact factor: 7.290
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