Literature DB >> 8052164

Evaluation of the sensitivity and specificity of polymerase chain reaction test kit, AMPLICOR Chlamydia trachomatis.

N Miyashita1, Y Lijima, A Matsumoto.   

Abstract

The sensitivity and specificity of the polymerase chain reaction (PCR) test kit, AMPLICOR Chlamydia trachomatis, were examined by the use of purified elementary bodies (EBs), cells having inclusions containing reticulate bodies alone and 20 clinical isolates. The numbers of EB and inclusion of C. trachomatis at the detection limit were determined to be approximately 2 to 4 EBs and one inclusion per assay, respectively. No reaction occurred for C. psittaci and C. pneumoniae. All clinical isolates were positively reacted in the PCR assay.

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Year:  1994        PMID： 8052164     DOI： 10.1111/j.1348-0421.1994.tb01747.x

Source DB:  PubMed          Journal:  Microbiol Immunol        ISSN： 0385-5600            Impact factor:   1.955


  8 in total

1.  Evaluation of the sensitivity and specificity of a ligase chain reaction test kit for the detection of Chlamydia trachomatis.

Authors:  N Miyashita; A Matsumoto; Y Niki; T Matsushima
Journal:  J Clin Pathol       Date:  1996-06       Impact factor: 3.411

2.  Differences in the sensitivity of the Amplicor Chlamydia trachomatis PCR assay.

Authors:  J M Ossewaarde; G J van Doornum; M Buimer; B Choueiri; A Stary
Journal:  Genitourin Med       Date:  1997-06

3.  Plaque formation by and plaque cloning of Chlamydia trachomatis biovar trachoma.

Authors:  A Matsumoto; H Izutsu; N Miyashita; M Ohuchi
Journal:  J Clin Microbiol       Date:  1998-10       Impact factor: 5.948

4.  Periodic health examination, 1996 update: 2. Screening for chlamydial infections. Canadian Task Force on the Periodic Health Examination.

Authors:  H D Davies; E E Wang
Journal:  CMAJ       Date:  1996-06-01       Impact factor: 8.262

5.  Diagnostic accuracy of a prototype point-of-care test for ocular Chlamydia trachomatis under field conditions in The Gambia and Senegal.

Authors:  Emma M Harding-Esch; Martin J Holland; Jean-François Schémann; Sandra Molina; Isatou Sarr; Aura A Andreasen; Chrissy h Roberts; Ansumana Sillah; Boubacar Sarr; Edward F Harding; Tansy Edwards; Robin L Bailey; David C W Mabey
Journal:  PLoS Negl Trop Dis       Date:  2011-08-02

6.  Impact of a single round of mass drug administration with azithromycin on active trachoma and ocular Chlamydia trachomatis prevalence and circulating strains in The Gambia and Senegal.

Authors:  Emma M Harding-Esch; Martin J Holland; Jean-François Schémann; Ansumana Sillah; Boubacar Sarr; Linus Christerson; Harry Pickering; Sandra Molina-Gonzalez; Isatou Sarr; Aura A Andreasen; David Jeffries; Chris Grundy; David C W Mabey; Bjorn Herrmann; Robin L Bailey
Journal:  Parasit Vectors       Date:  2019-10-22       Impact factor: 3.876

7.  Development and evaluation of a next-generation digital PCR diagnostic assay for ocular Chlamydia trachomatis infections.

Authors:  Chrissy H Roberts; Anna Last; Sandra Molina-Gonzalez; Eunice Cassama; Robert Butcher; Meno Nabicassa; Elizabeth McCarthy; Sarah E Burr; David C Mabey; Robin L Bailey; Martin J Holland
Journal:  J Clin Microbiol       Date:  2013-05-01       Impact factor: 5.948

8.  Field evaluation of the Cepheid GeneXpert Chlamydia trachomatis assay for detection of infection in a trachoma endemic community in Tanzania.

Authors:  Alexander Jenson; Laura Dize; Harran Mkocha; Beatriz Munoz; Jennifer Lee; Charlotte Gaydos; Thomas Quinn; Sheila K West
Journal:  PLoS Negl Trop Dis       Date:  2013-07-04
  8 in total

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