Literature DB >> 8045914

Cloning, expression, and molecular characterization of the gene encoding an extremely thermostable [4Fe-4S] ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus.

A Heltzel1, E T Smith, Z H Zhou, J M Blamey, M W Adams.   

Abstract

The gene for ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus was cloned, sequenced, and expressed in Escherichia coli. The coding region confirmed the determined amino acid sequence. Putative archaeon-type transcriptional regulatory elements were identified. The fdxA gene appears to be an independent transcriptional unit. Recombinant ferredoxin was indistinguishable from the protein purified from P. furiosus in its thermal stability and in the potentiometric and spectroscopic properties of its [4Fe-4S] cluster.

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Year:  1994        PMID： 8045914      PMCID： PMC196307          DOI： 10.1128/jb.176.15.4790-4793.1994

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  18 in total

1.  Elements of an archaeal promoter defined by mutational analysis.

Authors:  J Hain; W D Reiter; U Hüdepohl; W Zillig
Journal:  Nucleic Acids Res       Date:  1992-10-25       Impact factor: 16.971

2.  pBluescript II: gene mapping vectors.

Authors:  M A Alting-Mees; J M Short
Journal:  Nucleic Acids Res       Date:  1989-11-25       Impact factor: 16.971

Review 3.  Gene structure, organization, and expression in archaebacteria.

Authors:  J W Brown; C J Daniels; J N Reeve
Journal:  Crit Rev Microbiol       Date:  1989       Impact factor: 7.624

4.  Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli.

Authors:  E Amann; B Ochs; K J Abel
Journal:  Gene       Date:  1988-09-30       Impact factor: 3.688

5.  Mutational analysis of an archaebacterial promoter: essential role of a TATA box for transcription efficiency and start-site selection in vitro.

Authors:  W D Reiter; U Hüdepohl; W Zillig
Journal:  Proc Natl Acad Sci U S A       Date:  1990-12       Impact factor: 11.205

6.  Glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaebacterium Pyrococcus woesei: characterization of the enzyme, cloning and sequencing of the gene, and expression in Escherichia coli.

Authors:  P Zwickl; S Fabry; C Bogedain; A Haas; R Hensel
Journal:  J Bacteriol       Date:  1990-08       Impact factor: 3.490

7.  Spectroscopic characterization of the novel iron-sulfur cluster in Pyrococcus furiosus ferredoxin.

Authors:  R C Conover; A T Kowal; W G Fu; J B Park; S Aono; M W Adams; M K Johnson
Journal:  J Biol Chem       Date:  1990-05-25       Impact factor: 5.157

8.  A novel and remarkably thermostable ferredoxin from the hyperthermophilic archaebacterium Pyrococcus furiosus.

Authors:  S Aono; F O Bryant; M W Adams
Journal:  J Bacteriol       Date:  1989-06       Impact factor: 3.490

9.  Proton NMR investigation of the oxidized three-iron clusters in the ferredoxins from the hyperthermophilic archae Pyrococcus furiosus and Thermococcus litoralis.

Authors:  S C Busse; G N La Mar; L P Yu; J B Howard; E T Smith; Z H Zhou; M W Adams
Journal:  Biochemistry       Date:  1992-12-01       Impact factor: 3.162

10.  Transcription termination in the archaebacterium Sulfolobus: signal structures and linkage to transcription initiation.

Authors:  W D Reiter; P Palm; W Zillig
Journal:  Nucleic Acids Res       Date:  1988-03-25       Impact factor: 16.971
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  5 in total

1.  A novel phenanthrene dioxygenase from Nocardioides sp. Strain KP7: expression in Escherichia coli.

Authors:  A Saito; T Iwabuchi; S Harayama
Journal:  J Bacteriol       Date:  2000-04       Impact factor: 3.490

2.  Sequence, expression in Escherichia coli, and analysis of the gene encoding a novel intracellular protease (PfpI) from the hyperthermophilic archaeon Pyrococcus furiosus.

Authors:  S B Halio; I I Blumentals; S A Short; B M Merrill; R M Kelly
Journal:  J Bacteriol       Date:  1996-05       Impact factor: 3.490

3.  Regulation of iron metabolism by Pyrococcus furiosus.

Authors:  Yixuan Zhu; Sunil Kumar; Angeli L Menon; Robert A Scott; Michael W W Adams
Journal:  J Bacteriol       Date:  2013-03-15       Impact factor: 3.490

4.  Expression and in vitro assembly of recombinant glutamate dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus.

Authors:  J Diruggiero; F T Robb
Journal:  Appl Environ Microbiol       Date:  1995-01       Impact factor: 4.792

5.  Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei.

Authors:  Amir Ghasemi; Ali Hatef Salmanian; Nourkhoda Sadeghifard; Amir Ahmad Salarian; Mohammad Khalifeh Gholi
Journal:  Iran J Microbiol       Date:  2011-09
  5 in total

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