Literature DB >> 8039670

An improved method for detecting cytostatic toxin (emetic toxin) of Bacillus cereus and its application to food samples.

T Mikami1, T Horikawa, T Murakami, T Matsumoto, A Yamakawa, S Murayama, S Katagiri, K Shinagawa, M Suzuki.   

Abstract

We developed an improved HEp-2 cell assay method for the detection of Bacillus cereus toxin, which affects the proliferation of HEp-2 cells. The cytostatic toxin was stable upon exposure to heat, pH 2, pH 11 and trypsin, which suggests it is an emetic. Using the HEp-2 cell assay, we examined the distribution and contamination of B. cereus strains that produced an emetic toxin in various foods. Although there were 228 enterotoxin producers among 310 B. cereus strains obtained from foods, 16 of them produced the cytostatic type (emetic toxin). All of the strains that produced the cytostatic toxin were of the H.1 serotype.

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Year:  1994        PMID: 8039670     DOI: 10.1111/j.1574-6968.1994.tb06866.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN: 0378-1097            Impact factor:   2.742


  7 in total

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Authors:  Diego A Alonzo; T Martin Schmeing
Journal:  Protein Sci       Date:  2020-11-02       Impact factor: 6.725

2.  Rapid Ped-2E9 cell-based cytotoxicity analysis and genotyping of Bacillus species.

Authors:  Kristen M Gray; Padmapriya P Banada; Erin O'Neal; Arun K Bhunia
Journal:  J Clin Microbiol       Date:  2005-12       Impact factor: 5.948

3.  A novel sensitive bioassay for detection of Bacillus cereus emetic toxin and related depsipeptide ionophores.

Authors:  M A Andersson; R Mikkola; J Helin; M C Andersson; M Salkinoja-Salonen
Journal:  Appl Environ Microbiol       Date:  1998-04       Impact factor: 4.792

4.  Quantitative analysis of cereulide, the emetic toxin of Bacillus cereus, produced under various conditions.

Authors:  Max M Häggblom; Camelia Apetroaie; Maria A Andersson; Mirja S Salkinoja-Salonen
Journal:  Appl Environ Microbiol       Date:  2002-05       Impact factor: 4.792

5.  Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells.

Authors:  Jessica Minnaard; Vanessa Lievin-Le Moal; Marie-Helene Coconnier; Alain L Servin; Pablo F Pérez
Journal:  Infect Immun       Date:  2004-06       Impact factor: 3.441

6.  Detection and Isolation of Emetic Bacillus cereus Toxin Cereulide by Reversed Phase Chromatography.

Authors:  Eva Maria Kalbhenn; Tobias Bauer; Timo D Stark; Mandy Knüpfer; Gregor Grass; Monika Ehling-Schulz
Journal:  Toxins (Basel)       Date:  2021-02-04       Impact factor: 4.546

7.  Structures and function of a tailoring oxidase in complex with a nonribosomal peptide synthetase module.

Authors:  Camille Marie Fortinez; Kristjan Bloudoff; Connor Harrigan; Itai Sharon; Mike Strauss; T Martin Schmeing
Journal:  Nat Commun       Date:  2022-01-27       Impact factor: 14.919

  7 in total

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