| Literature DB >> 8037665 |
L Mahraoui1, J Takeda, J Mesonero, I Chantret, E Dussaulx, G I Bell, E Brot-Laroche.
Abstract
The effect of cyclic AMP on the expression of the fructose transporter, GLUT5, was studied in Caco-2 cells, a human colon cancer cell line that differentiates spontaneously in culture into cells with the properties of small intestine enterocytes. Treatment of differentiated Caco-2 cells with 50 microM forskolin, which stimulates adenylate cyclase and raises intracellular cyclic AMP levels, increased fructose uptake 2-fold and raised GLUT5 protein and mRNA levels 5- and 7-fold respectively. The increased GLUT5 mRNA levels in forskolin-treated cells are a result of stabilization of GLUT5 mRNA in these cells and increased transcription. The effect of cyclic AMP on GLUT5 transcription was assessed by measuring the activity of human GLUT5 promoter-reporter gene constructs in forskolin-treated differentiated Caco-2 cells. The results showed that forskolin stimulated the activity of the GLUT5-reporter gene constructs and this stimulatory effect was mediated by cis-acting regulatory sequences.Entities:
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Year: 1994 PMID： 8037665 PMCID： PMC1137157 DOI： 10.1042/bj3010169
Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857