Literature DB >> 803714

Reconstitution of Rh (D) antigen activity from human erythrocyte membranes solubilized by deoxycholate.

D J Lorusso, F A Green.   

Abstract

Proteins were selectively solubilized from human erythrocyte membranes with deoxycholate. After ultracentrifugation and preliminary fractionation procedures, the detergent was removed from the soluble membranes by Bio-Beads SM-2 and dialysis against 5 millimolar magnesium chloride. Reaggregation took place with the apparent formation of vesicles which showed serologically specific Rh antigen activity.

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Year:  1975        PMID： 803714     DOI： 10.1126/science.803714

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  6 in total

1.  Partial purification of Rho (D) antigen from Rh positive and negative erythrocytes.

Authors:  F V Plapp; M M Kowalski; L Tilzer; P J Brown; B J Evans; M Chiga
Journal:  Proc Natl Acad Sci U S A       Date:  1979-06       Impact factor: 11.205

Review 2.  Antigenicity, storage, and aging: physiologic autoantibodies to cell membrane and serum proteins and the senescent cell antigen.

Authors:  M M Kay; K Sorensen; P Wong; P Bolton
Journal:  Mol Cell Biochem       Date:  1982-11-26       Impact factor: 3.396

3.  Anti-Rho(D) IgG binds to band 3 glycoprotein of the human erythrocyte membrane.

Authors:  E J Victoria; L C Mahan; S P Masouredis
Journal:  Proc Natl Acad Sci U S A       Date:  1981-05       Impact factor: 11.205

4.  The identification of specific Rhesus-polypeptide-blood-group-ABH-active-glycoprotein complexes in the human red-cell membrane.

Authors:  S Moore; C Green
Journal:  Biochem J       Date:  1987-06-15       Impact factor: 3.857

5.  alpha-Methyldopa and the erythrocyte membrane.

Authors:  F A Green; C Y Jung; A Rampal; D J Lorusso
Journal:  Clin Exp Immunol       Date:  1980-06       Impact factor: 4.330

6.  Molecular characterization of the human red cell Rho(D) antigen.

Authors:  C G Gahmberg
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
  6 in total

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