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Literature DB >> 111249

Partial purification of Rho (D) antigen from Rh positive and negative erythrocytes.

F V Plapp, M M Kowalski, L Tilzer, P J Brown, B J Evans, M Chiga.

Abstract

A rapid method is described for partial purification of Rho(D) antigen from sodium deoxycholate-solubilized erythrocyte membranes by affinity chromatography on a column coupled with anti-Rho(D) IgG. The Rho(D) antigen is a low molecular weight membrane protein that comigrates with the lipid zone of erythrocytes during sodium dodecyl sulfate/polyacrylamide gel electrophoresis. Similar quantities of Rho(D) antigen were present in Rh positive and negative erythrocytes. However, in the latter erythrocytes the antigen may not be exposed to the external cell surface, explaining why these cells are not agglutinated by anti-Rho(D) antiserum. LW antigen was also present in the fraction containing Rho(D) antigen. The genetic implications of these findings are discussed.

Entities: Chemical Gene

Mesh：

Substances：

Year: 1979 PMID： 111249 PMCID： PMC383731 DOI： 10.1073/pnas.76.6.2964

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

14 in total

1. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.

Authors: F C GREENWOOD; W M HUNTER; J S GLOVER
Journal: Biochem J Date: 1963-10 Impact factor: 3.857

2. [CHAGAS' DISEASE AND BRUCELLOSIS. PRELIMINARY NOTE].

Authors: P P CORREIA
Journal: Rev Ginecol Obstet (Sao Paulo) Date: 1964-07

3. Glycoproteins of the CHO cell membrane: partial fractionation of the receptors for concanavalin A and wheat germ agglutinin using a lectin immunoprecipitation technique.

Authors: R L Juliano; G Li
Journal: Biochemistry Date: 1978-02-21 Impact factor: 3.162

4. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.

Authors: G Fairbanks; T L Steck; D F Wallach
Journal: Biochemistry Date: 1971-06-22 Impact factor: 3.162

5. Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate.

Authors: R T Swank; K D Munkres
Journal: Anal Biochem Date: 1971-02 Impact factor: 3.365

6. Glycoprotein receptors for concanavalin A isolated from pig lymphocyte plasma membrane by affinity chromatography in sodium deoxycholate.

Authors: D Allan; J Auger; M J Crumpton
Journal: Nat New Biol Date: 1972-03-08

5. The identification of specific Rhesus-polypeptide-blood-group-ABH-active-glycoprotein complexes in the human red-cell membrane.

Authors: S Moore; C Green
Journal: Biochem J Date: 1987-06-15 Impact factor: 3.857

6. The Rhesus D antigen. A dicyclohexylcarbodiimide-binding proteolipid.

Authors: P J Brown; J P Evans; L T Sinor; L T Tilzer; F V Plapp
Journal: Am J Pathol Date: 1983-02 Impact factor: 4.307

7. Molecular characterization of the human red cell Rho(D) antigen.

Authors: C G Gahmberg
Journal: EMBO J Date: 1983 Impact factor: 11.598

7 in total

Partial purification of Rho (D) antigen from Rh positive and negative erythrocytes.

1. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.

2. [CHAGAS' DISEASE AND BRUCELLOSIS. PRELIMINARY NOTE].

3. Glycoproteins of the CHO cell membrane: partial fractionation of the receptors for concanavalin A and wheat germ agglutinin using a lectin immunoprecipitation technique.

4. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.

5. Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate.

6. Glycoprotein receptors for concanavalin A isolated from pig lymphocyte plasma membrane by affinity chromatography in sodium deoxycholate.

7. Fluorometric assay of proteins in the nanogram range.

8. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

9. Antigen site densities and ultrastructural distribution patterns of red cell Rh antigens.

Review 10. The organization of proteins in the human red blood cell membrane. A review.

1. Exposure of the Rh0(D) antigen on the surface and cytoplasmic domains of the red cell membrane.

2. Anti-Rho(D) IgG binds to band 3 glycoprotein of the human erythrocyte membrane.

3. Oral administration of erythrocyte membrane antigen does not suppress anti-Rh(D) antibody responses in humans.

4. A monoclonal antibody for distinction of invasive and noninvasive clinical isolates of Entamoeba histolytica.

5. The identification of specific Rhesus-polypeptide-blood-group-ABH-active-glycoprotein complexes in the human red-cell membrane.

6. The Rhesus D antigen. A dicyclohexylcarbodiimide-binding proteolipid.

7. Molecular characterization of the human red cell Rho(D) antigen.