Literature DB >> 8034436

N-acetyl-B-D-glucosaminidase isoenzymes in the diagnosis of poisoning and kidney diseases.

Z Marchewka1, J Kuźniar, J Lembas-Bogaczyk, K Jacyszin.   

Abstract

Damaged lysosomes from renal tubular cells are, to the greatest degree, the source of activity of NAG in urine. Besides this, the enzyme can appear as a result of degranulation of granulocytes (PMN), active infection of the urinary system as also from serum as a result of glomerular filtration during damage to the glomerular basement membrane. For the purpose of explaining the source of origin of the enzyme in urine, NAG was separated into isoenzymes from the kidneys, granulocytes and serum for comparison with isoenzymes in physiological and pathological urines after ethylene glycol poisoning, and glomerulonephritis, respectively. The separation was made by column ion-exchange chromatography on DEAE-52 cellulose and by electrophoresis in 7% polyacrylamide gel. In addition, the thermostability of isolated isoenzymes was compared.

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Year:  1994        PMID： 8034436     DOI： 10.1007/bf02768292

Source DB:  PubMed          Journal:  Int Urol Nephrol        ISSN： 0301-1623            Impact factor:   2.370


  17 in total

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Journal:  Clin Chim Acta       Date:  1970-01       Impact factor: 3.786

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Journal:  Clin Chim Acta       Date:  1980-03-14       Impact factor: 3.786

5.  Urinary N-acetyl- beta-D-glucosaminidase activities in patients with renal disease.

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Journal:  Br Med J       Date:  1975-08-16

6.  Serum beta-hexosaminidase and alpha-mannosidase activities as markers of alcohol abuse.

Authors:  H Wehr; B Czartoryska; D Górska; H Matsumoto
Journal:  Alcohol Clin Exp Res       Date:  1991-02       Impact factor: 3.455

7.  Urinary N-acetyl-beta-D-glucosaminidase as an indicator of tubular damage in multiple myeloma.

Authors:  R A Coward; I W DeLamore; N P Mallick; L E Robinson
Journal:  Clin Chim Acta       Date:  1984-04-27       Impact factor: 3.786

8.  Increased serum and urinary N-acetyl-beta-D-glucosaminidase activity in human hypertension: early indicator of renal dysfunction.

Authors:  G Simon; S Morioka; D K Snyder
Journal:  Clin Exp Hypertens A       Date:  1984

9.  N-acetyl-beta-glucosaminidase and albuminuria in acromegaly.

Authors:  J Skrha; J Marek; J Srámková; P Stolba
Journal:  Horm Metab Res       Date:  1991-06       Impact factor: 2.936

10.  N-acetyl-beta-D-glucosaminidase (NAG) isoenzymes release from human monocyte-derived macrophages in response to zymosan and human recombinant interferon-gamma.

Authors:  R Bourbouze; F Raffi; G Dameron; H Hali-Miraftab; F Loko; J L Vilde
Journal:  Clin Chim Acta       Date:  1991-06-14       Impact factor: 3.786
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  2 in total

1.  Enzymuria and beta2-mikroglobulinuria in the assessment of the influence of proteinuria on the progression of glomerulopathies.

Authors:  Z Marchewka; J Kuźniar; A Długosz
Journal:  Int Urol Nephrol       Date:  2001       Impact factor: 2.370

2.  Enzymatic markers of cyclosporine nephrotoxicity in patients after renal transplantation.

Authors:  Z Marchewka; J Kuźniar; A Długosz
Journal:  Int Urol Nephrol       Date:  1999       Impact factor: 2.370
  2 in total

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