Literature DB >> 8032527

Immunoperoxidase staining of hepatitis C virus in formalin-fixed, paraffin-embedded needle liver biopsies.

T Uchida1, T Shikata, E Tanaka, K Kiyosawa.   

Abstract

The localization of hepatitis C virus (HCV) in the liver has not been well clarified. We report successful indirect immunoperoxidase staining of the HCV core antigen using polyclonal antibodies raised in rabbits and conventional formalin-fixed, paraffin-embedded needle biopsy sections of liver. The core antigen was distributed in a fine granular pattern diffusely, perisinusoidally, or focally within the hepatocellular cytoplasm of livers from patients with HCV infection. The staining tended to show a more heterogeneous pattern in terms of intensity and distribution in cases of more advanced disease. Hepatocellular carcinoma cells were also frequently stained. HCV immunostaining will provide important information on the pathogenesis and treatment of HCV-related liver diseases.

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Year:  1994        PMID： 8032527     DOI： 10.1007/bf00191430

Source DB:  PubMed          Journal:  Virchows Arch        ISSN： 0945-6317            Impact factor:   4.064


  17 in total

1.  Histological difference between complete responders and non-responders to interferon therapy of the livers of patients with chronic hepatitis C.

Authors:  T Uchida; M Taira; T Shikata; M Moriyama; N Tanaka; H Okubo; Y Arakawa
Journal:  Acta Pathol Jpn       Date:  1993-05

2.  Localization of hepatitis C virus (HCV) antigen by immunohistochemistry on fixed-embedded liver tissue.

Authors:  D Infantolino; F Bonino; A R Zanetti; R R Lesniewski; R Barbazza; M Chiaramonte
Journal:  Ital J Gastroenterol       Date:  1990-08

3.  Detection of hepatitis C virus RNA in the liver by in situ hybridization.

Authors:  Y Tanaka; N Enomoto; S Kojima; L Tang; M Goto; F Marumo; C Sato
Journal:  Liver       Date:  1993-08

4.  The nature and origin of proliferated bile ductules in alcoholic liver disease.

Authors:  T Uchida; R L Peters
Journal:  Am J Clin Pathol       Date:  1983-03       Impact factor: 2.493

5.  Histopathological analysis of chronic hepatitis B virus (HBV) infection in relation to HBV replication.

Authors:  K Suzuki; T Uchida; T Shikata
Journal:  Liver       Date:  1987-10

6.  Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis.

Authors:  N Kato; M Hijikata; Y Ootsuyama; M Nakagawa; S Ohkoshi; T Sugimura; K Shimotohno
Journal:  Proc Natl Acad Sci U S A       Date:  1990-12       Impact factor: 11.205

7.  Non-A, non-B hepatitis: visualization of virus-like particles from chimpanzee and human sera.

Authors:  K Abe; T Kurata; T Shikata
Journal:  Arch Virol       Date:  1989       Impact factor: 2.574

8.  Variable and hypervariable domains are found in the regions of HCV corresponding to the flavivirus envelope and NS1 proteins and the pestivirus envelope glycoproteins.

Authors:  A J Weiner; M J Brauer; J Rosenblatt; K H Richman; J Tung; K Crawford; F Bonino; G Saracco; Q L Choo; M Houghton
Journal:  Virology       Date:  1991-02       Impact factor: 3.616

9.  Hepatitis C virus antigen in hepatocytes: immunomorphologic detection and identification.

Authors:  K Krawczynski; M J Beach; D W Bradley; G Kuo; A M di Bisceglie; M Houghton; G R Reyes; J P Kim; Q L Choo; M J Alter
Journal:  Gastroenterology       Date:  1992-08       Impact factor: 22.682

10.  Detection of intrahepatic replication of hepatitis C virus RNA by in situ hybridization and comparison with histopathology.

Authors:  F Negro; D Pacchioni; Y Shimizu; R H Miller; G Bussolati; R H Purcell; F Bonino
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-15       Impact factor: 11.205
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  4 in total

1.  Liver histopathology in patients with concurrent chronic hepatitis C and HIV infection.

Authors:  E Bierhoff; H P Fischer; E Willsch; J Rockstroh; U Spengler; H H Brackmann; J Oldenburg
Journal:  Virchows Arch       Date:  1997-04       Impact factor: 4.064

2.  Intrahepatic cholangiocellular carcinoma and hepatocellular carcinoma developed after a 6-year sustained virological response to interferon therapy for chronic hepatitis C.

Authors:  Shinji Takashimizu; Sei-Ichiro Kojima; Junko Nagata; Yasuhiro Nishizaki; Tatehiro Kagawa; Koichi Shiraishi; Tetsuya Mine; Jun-Ichi Kaneko; Norihiro Kokudo; Norihito Watanabe
Journal:  Clin J Gastroenterol       Date:  2011-03-24

3.  Extensive biliary intraepithelial neoplasia (BilIN) and multifocal early intrahepatic cholangiocarcinoma in non-biliary cirrhosis.

Authors:  Anne-Laure Rougemont; Muriel Genevay; Thomas A McKee; Magali Gremaud; Gilles Mentha; Laura Rubbia-Brandt
Journal:  Virchows Arch       Date:  2010-04-29       Impact factor: 4.064

4.  Hepatitis C virus proteins modulate microRNA expression and chemosensitivity in malignant hepatocytes.

Authors:  Chiara Braconi; Nicola Valeri; Pierluigi Gasparini; Nianyuan Huang; Cristian Taccioli; Gerard Nuovo; Tetsuro Suzuki; Carlo Maria Croce; Tushar Patel
Journal:  Clin Cancer Res       Date:  2010-01-26       Impact factor: 12.531
  4 in total

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