AIMS: To examine persistent CD3-large granular lymphocytosis (LGL) cases for clonality, both by lineage specific (T cell receptor) and lineage independent (X-inactivation) molecular methods; and to find out whether X-inactivation studies are more appropriate than gene rearrangement studies for this subset of LGL disorders. METHODS: Patients were selected who had LGL of more than six months' duration and identified as CD3- by immunophenotyping. T cell receptor studies and, where possible, X-inactivation studies of the phosphoglycerate kinase (PGK) gene were carried out. Analysis of subpopulations was carried out on cases heterozygous for PGK by the use of a polymerase chain reaction (PCR) method for X-inactivation. RESULTS: Of 17 CD3- LGL cases studied, all were found to be germline for beta, gamma, and delta T cell receptor studies, and immunoglobulin heavy chain genes. However, six of these were analysed by X-inactivation of the PGK gene and two cases gave clonal band patterns but only within the CD3- subpopulation. CONCLUSIONS: Clonal analysis by the lineage independent method of X-inactivation allows clonal expansion undetected by T and B cell specific markers to be identified. It is therefore a more appropriate method for the analysis of CD3- LGL. This has implications for diagnosis in CD3- LGL disorders.
AIMS: To examine persistent CD3-large granular lymphocytosis (LGL) cases for clonality, both by lineage specific (T cell receptor) and lineage independent (X-inactivation) molecular methods; and to find out whether X-inactivation studies are more appropriate than gene rearrangement studies for this subset of LGL disorders. METHODS:Patients were selected who had LGL of more than six months' duration and identified as CD3- by immunophenotyping. T cell receptor studies and, where possible, X-inactivation studies of the phosphoglycerate kinase (PGK) gene were carried out. Analysis of subpopulations was carried out on cases heterozygous for PGK by the use of a polymerase chain reaction (PCR) method for X-inactivation. RESULTS: Of 17 CD3- LGL cases studied, all were found to be germline for beta, gamma, and delta T cell receptor studies, and immunoglobulin heavy chain genes. However, six of these were analysed by X-inactivation of the PGK gene and two cases gave clonal band patterns but only within the CD3- subpopulation. CONCLUSIONS: Clonal analysis by the lineage independent method of X-inactivation allows clonal expansion undetected by T and B cell specific markers to be identified. It is therefore a more appropriate method for the analysis of CD3- LGL. This has implications for diagnosis in CD3- LGL disorders.
Authors: I Stamenkovic; M Stegagno; K A Wright; S M Krane; E P Amento; R B Colvin; R J Duquesnoy; J T Kurnick Journal: Proc Natl Acad Sci U S A Date: 1988-02 Impact factor: 11.205
Authors: B Vogelstein; E R Fearon; S R Hamilton; A C Preisinger; H F Willard; A M Michelson; A D Riggs; S H Orkin Journal: Cancer Res Date: 1987-09-15 Impact factor: 12.701
Authors: Y Yoshikai; D Anatoniou; S P Clark; Y Yanagi; R Sangster; P Van den Elsen; C Terhorst; T W Mak Journal: Nature Date: 1984 Dec 6-12 Impact factor: 49.962
Authors: T H Rabbitts; A Stinson; A Forster; L Foroni; L Luzzatto; D Catovsky; L Hammarström; C I Smith; D Jones; A Karpas Journal: EMBO J Date: 1985-09 Impact factor: 11.598
Authors: Margarida Lima; Julia Almeida; Andrés García Montero; Maria dos Anjos Teixeira; Maria Luís Queirós; Ana Helena Santos; Ana Balanzategui; Alexandra Estevinho; Maria del Cármen Algueró; Paloma Barcena; Sónia Fonseca; Maria Luís Amorim; José Manuel Cabeda; Luciana Pinho; Marcos Gonzalez; Jesus San Miguel; Benvindo Justiça; Alberto Orfão Journal: Am J Pathol Date: 2004-10 Impact factor: 4.307