| Literature DB >> 2560238 |
S Houard1, C Hackel, A Herzog, A Bollen.
Abstract
Oligonucleotide primers were used to amplify specific DNA regions of the Bordetella pertussis genome by the polymerase chain reaction. One pair of primers, PTp1/PTp2, identified a 191-bp DNA fragment located in the regulatory region of the pertussis toxin operon; a second pair of primers led to amplification of a 121-bp DNA piece located in an insertion-like element specific to B. pertussis. Both sets of primers were able to discriminate between the pathogen and related Bordetella species; they detected down to 6 bacteria and appeared suitable for routine detection of B. pertussis in clinical specimens.Entities:
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Year: 1989 PMID： 2560238 DOI： 10.1016/0923-2508(89)90069-7
Source DB: PubMed Journal: Res Microbiol ISSN： 0923-2508 Impact factor: 3.992