Literature DB >> 8023741

The effect of methotrexate on lipoxygenase metabolism in neutrophils from rats: in vitro and ex vivo studies.

J S Hawkes1, L G Cleland, M J James.   

Abstract

Studies were undertaken to examine the effect of methotrexate (MTX) administered in vivo or added in vitro upon the production of the 5-lipoxygenase (5-LO) metabolites of arachidonic acid (AA) by rat neutrophils. Peptone-induced peritoneal exudate cells were stimulated by A23187 and the cell suspensions assayed for leukotriene B4 (LTB4), the all-trans isomers of LTB4 and 5-hydroxyeicosatetraenoic acid (5-HETE) using high-pressure liquid chromatography. MTX added in vitro to rat cells was weakly inhibitory; however, no inhibition of LTB4 production was seen following in vivo administration of MTX by oral, subcutaneous or intraperitoneal routes. On the basis of these findings, inhibition of 5-LO metabolism does not appear to explain the anti-inflammatory effects of MTX.

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Year:  1993        PMID： 8023741     DOI： 10.1007/bf01984059

Source DB:  PubMed          Journal:  Agents Actions        ISSN： 0065-4299


  16 in total

1.  Effect of low dose methotrexate on neutrophil chemotaxis induced by leukotriene B4 and complement C5a.

Authors:  C R Suarez; W C Pickett; D H Bell; D K McClintock; A L Oronsky; S S Kerwar
Journal:  J Rheumatol       Date:  1987-02       Impact factor: 4.666

2.  Methotrexate suppresses passive adjuvant arthritis: studies on the metabolism of methotrexate in mononuclear cells derived from normal and adjuvant arthritic rats.

Authors:  S C Ridge; K M Ferguson; N Rath; J Galivan; J H Freisheim; A L Oronsky; S S Kerwar
Journal:  J Rheumatol       Date:  1988-08       Impact factor: 4.666

3.  Restoration of diminished T-cell function in adjuvant induced disease by methotrexate: evidence for two populations of splenic T-cell suppressors.

Authors:  L Kourounakis; M A Kapusta
Journal:  J Rheumatol       Date:  1976-12       Impact factor: 4.666

4.  The effect of immunomodulating drugs on adjuvant-induced arthritis in Lewis rats.

Authors:  B D Jaffee; J S Kerr; E A Jones; J V Giannaras; M McGowan; N R Ackerman
Journal:  Agents Actions       Date:  1989-06

5.  Methotrexate inhibits macrophage activation as well as vascular and cellular inflammatory events in rat adjuvant induced arthritis.

Authors:  W J Johnson; M J DiMartino; P C Meunier; K A Muirhead; N Hanna
Journal:  J Rheumatol       Date:  1988       Impact factor: 4.666

6.  The effect of methotrexate on ex vivo lipoxygenase metabolism in neutrophils from patients with rheumatoid arthritis.

Authors:  J S Hawkes; L G Cleland; S M Proudman; M J James
Journal:  J Rheumatol       Date:  1994-01       Impact factor: 4.666

7.  Metabolic turnover of methotrexate polyglutamates in lysosomes derived from S180 cells. Definition of a two-step process limited by mediated lysosomal permeation of polyglutamates and activating reduced sulfhydryl compounds.

Authors:  J R Barrueco; D F O'Leary; F M Sirotnak
Journal:  J Biol Chem       Date:  1992-08-05       Impact factor: 5.157

8.  Interaction between fish and vegetable oils in relation to rat leucocyte leukotriene production.

Authors:  M J James; L G Cleland; R A Gibson; J S Hawkes
Journal:  J Nutr       Date:  1991-05       Impact factor: 4.798

9.  Efficacy of low-dose methotrexate in rheumatoid arthritis.

Authors:  M E Weinblatt; J S Coblyn; D A Fox; P A Fraser; D E Holdsworth; D N Glass; D E Trentham
Journal:  N Engl J Med       Date:  1985-03-28       Impact factor: 91.245

10.  Studies on the effect of methotrexate on macrophage function.

Authors:  S K Hu; Y L Mitcho; A L Oronsky; S S Kerwar
Journal:  J Rheumatol       Date:  1988-02       Impact factor: 4.666
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  2 in total

1.  The anti-inflammatory actions of methotrexate are critically dependent upon the production of reactive oxygen species.

Authors:  Darren C Phillips; Kevin J Woollard; Helen R Griffiths
Journal:  Br J Pharmacol       Date:  2003-02       Impact factor: 8.739

2.  The effect of a selective 5-lipoxygenase inhibitor, zileuton, on tissue damage in acute colonic inflammation in rats.

Authors:  A Zarif; D Eiznhamer; C Callaghan; M I Doria; L Broutman; A Keshavarzian
Journal:  Inflammation       Date:  1996-06       Impact factor: 4.092
  2 in total

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