| Literature DB >> 8022657 |
P A Majiwa1, R Thatthi, S K Moloo, J H Nyeko, L H Otieno, S Maloo.
Abstract
Relatively simple protocols employing non-radioactive DNA probes have been used for the detection of African trypanosomes in the blood of mammalian hosts and the saliva of live tsetse flies. In combination with the polymerase chain reaction (PCR), the protocols revealed trypanosomes in buffy-coat samples from antigenaemic but aparasitaemic cattle and in the saliva of live, infected tsetse flies. Furthermore, the protocols were used to demonstrate concurrent natural infections of single tsetse flies with different species of African trypanosomes.Entities:
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Year: 1994 PMID: 8022657 DOI: 10.1017/s0031182000076150
Source DB: PubMed Journal: Parasitology ISSN: 0031-1820 Impact factor: 3.234