Literature DB >> 8021199

Transformation of Actinomyces spp. by a gram-negative broad-host-range plasmid.

M K Yeung1, C S Kozelsky.   

Abstract

The gram-negative broad-host-range vector pJRD215 was transferred by electroporation into strains of Actinomyces viscosus or Actinomyces naeslundii at efficiencies which ranged from 10(2) to 10(7) transformants per microgram of plasmid DNA. The Actinomyces transformants expressed pJRD215-encoded resistance to kanamycin and streptomycin. Moreover, the transforming plasmid DNA had not undergone any deletions or rearrangements, nor had it integrated into the genomes of these strains.

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Year:  1994        PMID: 8021199      PMCID: PMC205619          DOI: 10.1128/jb.176.13.4173-4176.1994

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  20 in total

1.  An antigenic analysis of Lactobacillus acidophilus.

Authors:  C EFTHYMIOU; P A HANSEN
Journal:  J Infect Dis       Date:  1962 May-Jun       Impact factor: 5.226

2.  Vectors with restriction site banks. V. pJRD215, a wide-host-range cosmid vector with multiple cloning sites.

Authors:  J Davison; M Heusterspreute; N Chevalier; V Ha-Thi; F Brunel
Journal:  Gene       Date:  1987       Impact factor: 3.688

3.  Electroporation-induced transformation of intact cells of Clostridium perfringens.

Authors:  S P Allen; H P Blaschek
Journal:  Appl Environ Microbiol       Date:  1988-09       Impact factor: 4.792

4.  Immunochemical and functional studies of Actinomyces viscosus T14V type 1 fimbriae with monoclonal and polyclonal antibodies directed against the fimbrial subunit.

Authors:  J O Cisar; E L Barsumian; R P Siraganian; W B Clark; M K Yeung; S D Hsu; S H Curl; A E Vatter; A L Sandberg
Journal:  J Gen Microbiol       Date:  1991-08

5.  Simple and rapid method for isolating large plasmid DNA from lactic streptococci.

Authors:  D G Anderson; L L McKay
Journal:  Appl Environ Microbiol       Date:  1983-09       Impact factor: 4.792

6.  Method for the lysis of Gram-positive, asporogenous bacteria with lysozyme.

Authors:  B M Chassy; A Giuffrida
Journal:  Appl Environ Microbiol       Date:  1980-01       Impact factor: 4.792

7.  Transformation of Mycobacterium aurum and Mycobacterium smegmatis with the broad host-range gram-negative cosmid vector pJRD215.

Authors:  J Hermans; C Martin; G N Huijberts; T Goosen; J A de Bont
Journal:  Mol Microbiol       Date:  1991-06       Impact factor: 3.501

8.  Improved method for electroporation of Staphylococcus aureus.

Authors:  S Schenk; R A Laddaga
Journal:  FEMS Microbiol Lett       Date:  1992-07-01       Impact factor: 2.742

9.  Conservation of an Actinomyces viscosus T14V type 1 fimbrial subunit homolog among divergent groups of Actinomyces spp.

Authors:  M K Yeung
Journal:  Infect Immun       Date:  1992-03       Impact factor: 3.441

10.  Improved electroporation and cloning vector system for gram-positive bacteria.

Authors:  G M Dunny; L N Lee; D J LeBlanc
Journal:  Appl Environ Microbiol       Date:  1991-04       Impact factor: 4.792

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  14 in total

1.  Amended description of the genes for synthesis of Actinomyces naeslundii T14V type 1 fimbriae and associated adhesin.

Authors:  Ping Chen; John O Cisar; Sonja Hess; Jenny T C Ho; Kai P Leung
Journal:  Infect Immun       Date:  2007-05-07       Impact factor: 3.441

2.  Sortase-catalyzed assembly of distinct heteromeric fimbriae in Actinomyces naeslundii.

Authors:  Arunima Mishra; Asis Das; John O Cisar; Hung Ton-That
Journal:  J Bacteriol       Date:  2007-02-02       Impact factor: 3.490

3.  Synthesis and function of Actinomyces naeslundii T14V type 1 fimbriae require the expression of additional fimbria-associated genes.

Authors:  M K Yeung; P A Ragsdale
Journal:  Infect Immun       Date:  1997-07       Impact factor: 3.441

4.  Lethality of sortase depletion in Actinomyces oris caused by excessive membrane accumulation of a surface glycoprotein.

Authors:  Chenggang Wu; I-Hsiu Huang; Chungyu Chang; Melissa Elizabeth Reardon-Robinson; Asis Das; Hung Ton-That
Journal:  Mol Microbiol       Date:  2014-09-17       Impact factor: 3.501

5.  Roles of fructosyltransferase and levanase-sucrase of Actinomyces naeslundii in fructan and sucrose metabolism.

Authors:  L J Bergeron; R A Burne
Journal:  Infect Immun       Date:  2001-09       Impact factor: 3.441

6.  Characterization of the fructosyltransferase gene of Actinomyces naeslundii WVU45.

Authors:  L J Bergeron; E Morou-Bermudez; R A Burne
Journal:  J Bacteriol       Date:  2000-07       Impact factor: 3.490

7.  Analysis of urease expression in Actinomyces naeslundii WVU45.

Authors:  E Morou-Bermudez; R A Burne
Journal:  Infect Immun       Date:  2000-12       Impact factor: 3.441

8.  Identification of a gene involved in assembly of Actinomyces naeslundii T14V type 2 fimbriae.

Authors:  M K Yeung; J A Donkersloot; J O Cisar; P A Ragsdale
Journal:  Infect Immun       Date:  1998-04       Impact factor: 3.441

9.  Construction and use of integration plasmids to generate site-specific mutations in the Actinomyces viscosus T14V chromosome.

Authors:  M K Yeung
Journal:  Infect Immun       Date:  1995-08       Impact factor: 3.441

10.  Genetic and physiologic characterization of urease of Actinomyces naeslundii.

Authors:  E Morou-Bermudez; R A Burne
Journal:  Infect Immun       Date:  1999-02       Impact factor: 3.441

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