Literature DB >> 8020060

Location of PHM/VIP mRNA in human gastrointestinal tract detected by in situ hybridization.

H E Bredkjaer1, B S Wulff, P C Emson, J Fahrenkrug.   

Abstract

The expression of the gene for vasoactive intestinal polypeptide (VIP) and peptide histidine methionine (PHM) in the human gastrointestinal tract was studied by in situ hybridization and Northern blotting for PHM/VIP mRNA and immunocytochemistry using specific antisera against the bioactive peptides PHM and VIP. In the colon sigmoideum, antisera against all five putative processing products of the VIP precursor (prepro-VIP) were used, namely prepro-VIP 22-79, PHM, prepro-VIP 111-122, VIP and prepro-VIP 156-170. Furthermore, RNA extracted from various regions of the gastrointestinal tract was examined by Northern blots and hybridization to a VIP-cDNA probe. Throughout the gastrointestinal tract, PHM/VIP mRNA was found in neurons only. Using single- or double-staining methods, we demonstrated both PHM/VIP mRNA and the corresponding peptides PHM and VIP in the neurons. In the sigmoideum, the single-staining methods were extended to investigate whether the neurons simultaneously contained PHM/VIP mRNA and each of the five prepro-VIP-derived peptides. Only one major band of PHM/VIP mRNA (1.9 kb) was found by Northern blotting in the tissue of the gastrointestinal tract.

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Year:  1994        PMID： 8020060     DOI： 10.1007/bf00306108

Source DB:  PubMed          Journal:  Cell Tissue Res        ISSN： 0302-766X            Impact factor:   5.249


  31 in total

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Authors:  H E Bredkjaer; D Rønnov-Jessen; L Fahrenkrug; E Ekblad; J Fahrenkrug
Journal:  Regul Pept       Date:  1991-04-25

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Authors:  I Gozes; E Giladi; Y Shani
Journal:  J Neurochem       Date:  1987-04       Impact factor: 5.372

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Authors:  V Mutt; S I Said
Journal:  Eur J Biochem       Date:  1974-03-01

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Authors:  S I Said; V Mutt
Journal:  Nature       Date:  1970-02-28       Impact factor: 49.962

5.  Combination of immunocytochemistry and in situ hybridization in the same tissue section of rat pituitary.

Authors:  B D Shivers; R E Harlan; D W Pfaff; B S Schachter
Journal:  J Histochem Cytochem       Date:  1986-01       Impact factor: 2.479

6.  Distribution of Tyrosine Hydroxylase mRNA in the Rat Central Nervous System Visualized by Alkaline Phosphatase in situ Hybridization Histochemistry.

Authors:  H. Kiyama; P. C. Emson; J. Ruth
Journal:  Eur J Neurosci       Date:  1990       Impact factor: 3.386

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Journal:  Nature       Date:  1982-07-15       Impact factor: 49.962

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Journal:  J Am Chem Soc       Date:  1974-07-24       Impact factor: 15.419

9.  Complete nucleotide sequence of human vasoactive intestinal peptide/PHM-27 gene and its inducible promoter.

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Journal:  Ann N Y Acad Sci       Date:  1988       Impact factor: 5.691

10.  Tyrosine 3-hydroxylase in rat brain and adrenal medulla: hybridization histochemistry and immunohistochemistry combined with retrograde tracing.

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Journal:  Proc Natl Acad Sci U S A       Date:  1986-08       Impact factor: 11.205
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  3 in total

Review 1.  Regulation of VIP gene expression in general. Human lung cancer cells in particular.

Authors:  A Davidson; T W Moody; I Gozes
Journal:  J Mol Neurosci       Date:  1996       Impact factor: 3.444

2.  Functional coupling between nitric oxide synthesis and VIP release within enteric nerve terminals of the rat: involvement of protein kinase G and phosphodiesterase 5.

Authors:  M Kurjak; R Fritsch; D Saur; V Schusdziarra; H D Allescher
Journal:  J Physiol       Date:  2001-08-01       Impact factor: 5.182

3.  The topography, architecture and structure of the enteric nervous system in the jejunum and ileum of cattle.

Authors:  O B Balemba; G K Mbassa; W D Semuguruka; R J Assey; C K Kahwa; A Hay-Schmidt; V Dantzer
Journal:  J Anat       Date:  1999-07       Impact factor: 2.610
  3 in total

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